| 楤木皂苷A在大鼠体内的组织分布研究 |
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| 引用本文： | 郭东艳,翟秉涛,吕杨,史亚军,范妤,王露,王媚.楤木皂苷A在大鼠体内的组织分布研究[J].中国中药杂志,2017,42(20):4002-4006. |
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| 作者姓名： | 郭东艳 翟秉涛 吕杨 史亚军 范妤 王露 王媚 |
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| 作者单位： | 陕西省中药基础与新药研究重点实验室, 陕西 西安 712046;陕西中医药大学, 陕西 西安 712046,陕西中医药大学, 陕西 西安 712046,陕西中医药大学, 陕西 西安 712046,陕西省中药基础与新药研究重点实验室, 陕西 西安 712046;陕西中医药大学, 陕西 西安 712046,陕西中医药大学, 陕西 西安 712046,陕西省中药基础与新药研究重点实验室, 陕西 西安 712046;陕西中医药大学, 陕西 西安 712046,陕西中医药大学, 陕西 西安 712046 |
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| 基金项目： | 国家自然科学基金项目（81703925）；陕西省科技厅科技统筹项目（2013KTCQ03-12） |
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| 摘 要： | 楤木皂苷A为太白楤木中的主要活性成分之一。该文通过建立SD大鼠主要脏器中楤木皂苷A的LC-MS/MS分析方法,同时口服灌胃楤木皂苷A 50 mg·kg~(-1),测定大鼠主要脏器(心、肝、脾、肺、肾、脑)中的楤木皂苷A含量,探讨体内的组织分布特征。结果显示楤木皂苷A在SD大鼠主要脏器中的方法学考察符合要求,口服灌胃楤木皂苷A 50 mg·kg~(-1),在心、肝、脾、肺、肾、脑组织均有分布且在1 h或2 h时达到最大值。在给药后不同时间点楤木皂苷A的组织分布情况不同:给药20min各组织含量:肝心脾肺肾脑;给药1 h各组织含量:肝脾肾肺心脑;给药2 h各组织含量:肝肾心脾肺脑;给药4 h各组织含量:肾肝脾心肺脑;给药8 h各组织含量:脾心肝肾肺脑。提示楤木皂苷A主要分布于肝组织,这也与太白楤木常用于治疗肝脏疾病具有一定的相关性。另外,楤木皂苷A在脑组织中虽含量低但有明显分布,提示药物可能会透过血脑屏障,也为楤木皂苷A在脑组织的研究提供了依据。
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| 关 键 词： | 楤木皂苷A 组织分布 HPLC-MS/MS |
| 收稿时间： | 2017/6/28 0:00:00 |
Tissue distribution of araloside A in rats |
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| GUO Dong-yan,ZHAI Bing-tao,LV Yang,SHI Ya-jun,FAN Yu,WANG Lu and WANG Mei.Tissue distribution of araloside A in rats[J].China Journal of Chinese Materia Medica,2017,42(20):4002-4006. |
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| Authors: | GUO Dong-yan ZHAI Bing-tao LV Yang SHI Ya-jun FAN Yu WANG Lu and WANG Mei |
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| Institution: | Shaanxi Key Laboratory of Basic and New Herbal Medicament Research, Xi''an 712046, China;Shaanxi University of Chinese Medicine, Xi''an 712046, China,Shaanxi University of Chinese Medicine, Xi''an 712046, China,Shaanxi University of Chinese Medicine, Xi''an 712046, China,Shaanxi Key Laboratory of Basic and New Herbal Medicament Research, Xi''an 712046, China;Shaanxi University of Chinese Medicine, Xi''an 712046, China,Shaanxi University of Chinese Medicine, Xi''an 712046, China,Shaanxi Key Laboratory of Basic and New Herbal Medicament Research, Xi''an 712046, China;Shaanxi University of Chinese Medicine, Xi''an 712046, China and Shaanxi University of Chinese Medicine, Xi''an 712046, China |
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| Abstract: | Araloside A is one of the main active ingredients of Aralia taibaiensis. In this study, HPLC-MS/MS analysis method of araloside A in the main organs of SD rats was established. At the same time, the content of araloside A in the main organs (heart, liver, spleen, lung, kidney, brain) after oral administration with araloside A (50 mg·kg-1) were determined to explore the tissue distribution characteristics of araloside A in vivo. The results showed that the methodological study of araloside A in the main organs of SD rats met the requirements, araloside A distributed in heart, liver, spleen, lung, kidney and brain tissues reached peak at 1 h or 2 h after oral administration with 50 mg·kg-1.The distributions of araloside A at different time points after administration were distinct as follows:the content of araloside A at 20 min:liver>heart>spleen>lung>kidney>brain; the content of araloside A at 1 h:liver > spleen > kidney > lung > heart > brain; the content of araloside A at 2 h:liver > kidney > heart > spleen > lung > brain; the content of araloside A at 4 h:kidney > liver > spleen > heart > lung > brain; the content of araloside A at 8 h:spleen > heart > liver > kidney > lung > brain. Therefore, araloside A was mainly distributed in liver tissue, which had a certain correlation with the common use of Aralia taibaiensis in the treatment of hepatic disease. In addition, araloside A shows a low content but an obvious distribution in brain tissues, which indicates that the drug can pass through blood-brain barrier, and provides the basis for the study of araloside A in brain tissue. |
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| Keywords: | araloside A tissue distribution HPLC-MS/MS |
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