骨关节炎患者膝关节软骨和滑膜中p38MAPK、NF-κB的表达及其意义

①目的探讨骨关节炎（Osteoarthritis，OA）中p38丝裂原活化蛋白激酶（p38MAPK）、核转录因子-κB（NF-κB）蛋白的表达及其意义。②方法采用免疫组织化学技术检测60倒OA患者及20例正常对照组膝关节软骨与滑膜中磷酸化p38（P-p38）、NF-κBp65的表选，并进行相关性分析。③结果P-p38、NF-κBp65蛋白在实验组阳性表达的积分光密度（IOD）值均显著高于正常对照组（P〈0．01）实验组P-p38及NF-κBp65蛋白表达成正相关（r=0．426，P〈0．01）。④结论p38MAPK及NF-κB信号转导通路在OA关节软骨和滑膜细胞中被激活，P-p38的高表达可能促进了NF-κB的表达，二者对OA的发生发展起重要作用。

Expression and significance of p38 mitogen - activated protein kinase,nuclear factor - kappa B in knee articular cartilage and synovium of osteoarthritis patients

Objective To investigate the significance of expression of p38MAPK, NF -κB protein in Osteoarthritis. Meth- ods Immunohistochemical analysis were used to determine the expression of p38MAPK and NF-κB p65 protein in chondrocytes and synoviocytes of osteoarthritis from 60 patients with osteoarthritis groups and 20 patients with normal control groups, as well as their correlations in osteoarthr/tis. Results IOD of P - p38 and NF -κB p65 protein in the experimental group was significantly higher than normal control group（ P 〈 0.01 ）. There was a positive correlation between the expression of P - p38 protein and NF -κB p65 protein （r = 0. 426 ,P 〈 0.01 ）in the experimental group. Conelusion The signal transduction pathway of p38MAPK and NF -κB are activated in the chondrocytes and synoviocytes of OA. The higher expression of P - p38 may promote the expression of NF -κB of chondrocytes and synoviocytes in OA, and both accelerate the biological behavior of OA such as occurrence and pro- gress , etc.