IGF-1对骨髓基质细胞分泌BMP-2和VEGF的影响

目的 :研究不同浓度的胰岛素样生长因子 1(IGF -1)对骨髓基质细胞 (MSC)分泌骨形成蛋白 -2(BMP -2 )和血管内皮细胞生长因子 (VEGF)的影响。方法 :培养大鼠骨髓基质细胞 ,对第三代细胞分别用 5 0ng/ml,2 5ng/ml ,12 .5ng/ml,6.2 5ng/ml,3 .12 5ng/ml的IGF -1进行培养 ,以正常培养的MSC为对照组 ,分别于1、3、5、7d对MSC进行VEGF或BMP -2免疫组化观察。通过图像分析观测MSC分泌BMP -2和VEGF的变化。结果 :IGF -1对BMP -2的分泌有一定的促进作用。IGF -1可以明显促进MSC表达VEGF ,以 5 0ng/ml合成作用最明显 ,IGF -1促进MSC分泌VEGF和BMP -2具有时间及浓度依赖性。结论 :IGF -1可明显地促进MSC分泌BMP -2和VEGF。

Regulation of VEGF and BMP-2 expression in bone marrow stem cell by IGF-I

Objective:To evaluate regulation the expression of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) in bone marrow stem cell (MSC) by different concentrations of Insulin-like growth factor I (IGF-1). Method: Culture the rat 's bone marrow stem cells (MSC). The cells were treated with varying concentrations of hIGF-I (50, 25, 12.5, 6.25 and 3.125 ng/ml) in 2% BS. To study the response over time, cells were treated with hIGF-I (100 ng/ml) for 1, 3, 5 and 7d. Immunohistochemistry methods were used to investigate the ability of synthesis of BMP-2 and VEGF of MSC. Then, the results of immunohistochemistry were analyzed with HPIAS-1000 color image analyse system.Result: IGF-1 promoted the ability of synthesis of VEGF and BMP-2. Induction of VEGF was seen at 50 ng/ml IGF-I in the early time. The concentrations of IGF-1 and the time affect the VEGF and BMP-2 synthesis.Conclusion: the results suggest that the certain concentrations of IGF-1 can promote the synthesis of VEGF and BMP-2.