| 毛细管电泳PCR-SSCP分析p16基因突变 |
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| 引用本文： | 吴逸明,张振中,史香林. 毛细管电泳PCR-SSCP分析p16基因突变[J]. 郑州大学学报(医学版), 2000, 35(3): 236 |
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| 作者姓名： | 吴逸明 张振中 史香林 |
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| 作者单位： | Department of Occupational Health and Health Toxicology, Henan Medical University Zhengzhou 450052 P.R.China,Department of Pharmacy, Henan Medical University, Zhengzhou 450052 P.R.China,Pathology and Physiology Research, NIOSH, Morgantown, WV 26505, USA |
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| 基金项目： | 国家自然科学基金，204022300， |
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| 摘 要： | Lung cancer is one of the most common cancers in the world. Some genetic alterations such as p53 gene and ras gene mutations, have been identified in this disease. Recently, a putative tumor suppressor gene, the p16/CDKN2/MTS1 gene containing 3 extrons and 2 introns, located in the chromosome p21 region, was cloned independently by three research groups. Traditionally, gene mutation analysis was performed by slab polyacrylamide gel electrophoresis. However, this method is laborious, time-consuming, low sensitivity and harmful to human health. Capillary electrophoresis (CE) with the characteristics of rapidity and high performance has numerous advantages over conventional slab polyacrylamide gel electrophoresis. An important advantage of CE is that the commercially available system is automation.
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| 关 键 词： | p16 gene gene mutation SSCP high performance capillary electrophoresis PCR |
PCR-SSCP analysis of p16 gene mutation by capillary electrophoresis with laser-induced fluorescence detector |
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| WU Yiming,ZHANG Zhenzhong,SHI Xianglin. PCR-SSCP analysis of p16 gene mutation by capillary electrophoresis with laser-induced fluorescence detector[J]. Journal of Zhengzhou University: Med Sci, 2000, 35(3): 236 |
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| Authors: | WU Yiming ZHANG Zhenzhong SHI Xianglin |
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| Abstract: | Lung cancer is one of the most common cancers in the world. Some genetic alterations such as p53 gene and ras gene mutations, have been identified in this disease. Recently, a putative tumor suppressor gene, the p16/CDKN2/MTS1 gene containing 3 extrons and 2 introns, located in the chromosome p21 region, was cloned independently by three research groups. Traditionally, gene mutation analysis was performed by slab polyacrylamide gel electrophoresis. However, this method is laborious, time-consuming, low sensitivity and harmful to human health. Capillary electrophoresis (CE) with the characteristics of rapidity and high performance has numerous advantages over conventional slab polyacrylamide gel electrophoresis. An important advantage of CE is that the commercially available system is automation. |
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| Keywords: | p16 gene gene mutation SSCP high performance capillary electrophoresis PCR |
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