百令胶囊对小鼠树突细胞影响的实验研究

目的与经典免疫抑制剂做比较，了解冬虫夏草在体外对树突细胞的分化、成熟及功能的影响，进一步研究冬虫夏草的免疫抑制作用机理。方法百令胶囊提取液、他克莫司（FK506）和雷帕霉素（Ra-pamycin，Rapa）分别与小鼠髓源性树突细胞培养。流式细胞仪分析免疫抑制剂对树突细胞表型的影响。经过与不同药物培养的树突细胞作为刺激细胞与异体淋巴细胞进行混和培养，观察树突细胞刺激增殖能力的变化。结果百令胶囊在体外对树突细胞表型标记物及共刺激分子没有影响，与他克莫司差异无显著性。雷帕酶素可以降低树突细胞表型标记物及共刺激分子的表达；对白细胞增殖的刺激作用，百令胶囊有明显的抑制效果，并表现为剂量依赖。结论百令胶囊可以影响树突细胞刺激增殖的能力，其机理可能是通过抑制抗原递呈细胞的功能，阻断外来信号刺激的呈递，达到免疫低反应状态，最终实现免疫抑制的效果。

Experimental Study on Effect of Bailing Capsule on Dendritic Cells in Mice

Objective Comparing with the classic immuno-supressors, to probe in the in vitro effect of Cordyceps Sinensis (CS) on the differentiation, maturation and function of dentritic cells (DCs), and further to explore its mechanism. Methods Mice myeloid DCs were cultured respectively with extraction of Bailing Capsule (CSE), a Chinese medical preparation made of CS, Rapamycin and Tacrolimus, and the effect of various drugs on phenotype of DCs was analyzed with flow cytometer. Then, using as the stimulator, the DCs cultured with different drugs were mixed and cultured with heterogenous lymphocytes for observing the stimulating capacity of DCs on cell proliferation. Results CSE showed no in vitro effect on phenotype markers and co-stimulation molecules of DCs, the difference between CSE and Tacrolimus was insignificant, while Rapamycin could reduce the two parameters. CSE showed a marked suppressive effect on DCs in stimulating leucocyte proliferation in a dose-dependent manner. Conclusion CSE could affect the stimulating capacity of DCs on cell proliferation, which is probably by means of inhibiting the function of antigen presentating cells to block the presentation of extrinsic signal, and make the low immune response condition, thus to obtain the effect of immunosuppression.