C57-TgN(HBVadr2.0)SMMU小鼠血清和肝组织中HBsAg的表达模式分析

目的:分析C57-TgN(HBVadr2.0)SMMU"3号"品系转基因小鼠的月龄、性别和遗传背景与其血清和肝组织中HBsAg表达模式的关系.方法:采用血清ELISA检测和免疫组织化学的方法检测转基因小鼠血清和肝组织中HBsAg的表达,统计学方法分析转基因小鼠血清和肝组织中HBsAg的表达与小鼠的月龄、性别、遗传背景的相关性.结果:转基因小鼠在出生后1～3个月时,血清中HBsAg的表达较低或甚至不表达,8个月龄左右时最高,此后逐渐降低,但肝组织中HBsAg的表达相对稳定在较高水平,能真实反映转基因小鼠中HBsAg的表达.雄性小鼠血清和肝组织中HBsAg的表达量高于雌性小鼠(P＜0.05).转基因小鼠与C57BL/6品系、DAB品系和129s品系正常小鼠交配后的子代,其血清和肝组织中HBsAg表达量均未发生变化.血清阳性的小鼠中肝组织HBsAg阳性率为94.05%,两者呈正相关关系(r=0.257,P＜0.01).结论:C57-TgN(HBVadr2.0)SMMU"3号"品系转基因小鼠血清和肝组织中HBsAg的表达具有发育调节和性别差异.

Expression pattern of HBsAg in serum and liver tissue of C57-TgN(HBVadr2.0)SMMU transgenic mice

Objective:To explore the relationship between expression pattern of HBsAg in the serum and liver tissue and age, sex and genetic background of C57-TgN(HBVadr2.0)SMMU3 transgenic mice. Methods: ELISA and immunohistochemistry were used to evaluate the expression level of HBsAg in serum and liver of C57-TgN(HBVadr2.0)SMMU3 transgenic mice. The relationship between expression pattern and age, sex and genetic background was analyzed by statistical methods. Results: One to 3 months old transgenic mice expressed a low level or no HBsAg; expression of HBsAg reached a peak level in 8 months old mice and then declined. Expression of HBsAg in the liver was more stable than that in serum,which represented the real expression level of HBsAg in transgenic mice. Expression levels of HBsAg in serum and liver of male transgenic mice were higher than those in female mice (P<0.05). The expression levels of HBsAg had no changes in the offsprings of different genetic background mice. There were 94.05% transgenic mice expressed both HBsAg in serum and HBsAg in liver tissue,and they had a positive correlation(r=0.257,P<0.01). Conclusion: Expression of HBsAg has the characteristics of developmental regulation and sexual dimorphism in serum and liver of C57-TgN(HBVadr2.0)SMMU3 transgenic mice.