重组人粒细胞集落刺激因子rhG—CSF的纯化工艺探讨 |
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引用本文: | 黄健,王永存,江敏.重组人粒细胞集落刺激因子rhG—CSF的纯化工艺探讨[J].黑龙江医药,2002,15(1):8-9. |
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作者姓名: | 黄健 王永存 江敏 |
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作者单位: | 1. 哈药集团技术中心 2. 哈尔滨生化二厂 |
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摘 要: | 粒细胞集落刺激因子(granulocyte colony stimulating factor G-CSF)主要来源于巨嗜细胞,内皮细胞及纤维母细胞。能高度特异性的刺激中性白细胞系的功能活化因子。在这里我们对rhG-CSF的纯化工艺进行探讨。通常对rhG-CSF的纯化采用S-200和DE柱进行纯化,在这里我们采用另外一种方法,即采用Q柱和CM柱进行纯化。并对其比较讨论。
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关 键 词: | 粒细胞集落刺激因子 纯化 |
修稿时间: | 2001年7月23日 |
The Pure Study of Recominant Human Granulocyte Colony Stimulating Factor |
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Abstract: | The granulocyte colony stimulating factor mainly come from macrophagocyle, en- dothelial cell and fibroblast. It can highly specially stimuate functional active factor of neutral leukocyte strain, here, we want to discuss the technics about purification of rhG-CSF. usually want to purity rhG-CSF by S-200 pole and DE pole. here, we want to adopt another way, name- ly adopt Q pole and CM pole to purity it. Then compare and discuss them. |
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Keywords: | granulocyte colony stimulating factor G-CSF purity |
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