GM-CSF 凝胶对 SD 大鼠深 Ⅱ度烧伤创面溶痂的影响

目的：观察重组人粒细胞巨噬细胞集落刺激因子（ rhGM-CSF）对深Ⅱ度烧伤创面坏死组织溶痂和促进创面愈合的作用效果。方法 SD大鼠70只用热液烧伤的方法（75℃、8 s）制成背部深Ⅱ度烧伤创面，烧伤大鼠随机分为实验组和对照组（每组35只）。对照组（ C组）：创面局部外用不含rhGM-CSF的凝胶基质，实验组（ E组）：创面局部外用rhGM-CSF凝胶（100μg/10 g）。分别于制创后1、3、5、7、10、14和21 d观察2组动物创面情况并摄像，记录创面溶痂时间；用图像分析软件计算不同时相点的溶痂率；并于不同的时相点取创面组织， HE染色观察创面组织形态及修复情况。结果从烧伤后第5天起各时相点，实验组大鼠创面溶痂率与对照组表现出差异；实验组创面溶痂时间为（10．73±2．47）d较对照组（14．26±2．65）d显著缩短（P＜0．01）；实验组和对照组创面愈合时间分别为（16．21±1.27）d和（18．05±1．36）d，差异有非常显著性（P＜0．01）。结论外源性rhGM-CSF的应用可促进深II度烧伤创面溶痂，从而促进深II度烧伤创面愈合。

Effect of recombinant human granulocyte-macrophage colony stimulating factor gel on melting crust in deep partial thickness scalding rats

Objective To explore the effect of recombinant human granulocyte-macrophage colony-stimulating factor （ rh-GM-CSF） gel on crust melting and wound healing of deep partial thickness scalding SD rats .Methods A total of 70 SD rats were scalded on the back using the method of thermal-water burns at 75℃for 8s.A deep partial-thickness burn was created in all the rats.The scalded rats were randomly divided into two groups （35 for each group）.The control group（C） was treated with gel matrix while the experiment group （ E） was treated with rhGM-CSF gel .The wounds treatment of the two groups lasted 21 days.Observations of the wounds were made by photograph at various time-points.In addition, the removal time of wound eschar in all the rats was recorded .The percentages of removal area in the wounds were calculated .The scalding wound speci-mens were obtained at different time points .Re-epithelization was observed by hematoxylin and eosin staining .Results The removal-area percentage of eschar in the experiment group was significantly higher than that in the control group from Day 5 post-injury.The average removal-time of eschar in the experiment group was 10.73 ±2.47d,which was much shorter than that in the control group＇s 14.26 ±2.65d（P〈0.01）.The time of wound healing in the experiment group was 16.73 ± 1.27d,which was significantly shorter than that in the control group＇s 18.05 ±1.36d（P〈0.01）.Conclusion RhGM-CSF gel may promote debridement and wound healing in deep partial thickness burn .