| 免疫磁珠法分选胚胎大鼠脑神经干细胞的初步研究 |
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| 引用本文： | 高国一 李莉. 免疫磁珠法分选胚胎大鼠脑神经干细胞的初步研究[J]. 第二军医大学学报, 2000, 21(11): 1092-1094 |
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| 作者姓名： | 高国一 李莉 |
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| 作者单位： | [1]第二军医大学长征医院神经外科,上海 [2]长征医院临床实验科,上海 |
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| 基金项目： | 上海市科技发展基金资助项目 ( 9941190 89) |
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| 摘 要： | 目的：探索应用免疫磁珠间接阳性法分选神经上皮干细胞蛋白（ｎｅｓｔｉｎ）阳性的神经干细胞群的实验条件，为研究神经干细胞的特性与神经干细胞的培养和移植研究创造有利条件。方法：制取胎鼠大脑组织细胞悬液，免疫磁珠法分选胎鼠脑神经干细胞，以流式细胞术检测阳性细胞纯度，以锥虫蓝染色法检测细胞活性。结果：该法分选的ｎｅｓｔｉｎ阳性细胞纯度为９３．０％～９９．７％，其中活性细胞为９２％～９７％。结论：免疫磁珠法分离胎鼠脑神经干细胞群落简便、有效，可以为神经干细胞细胞培养和移植提供细胞来源，也为研究高纯度神经干细胞的特性提供了实验基础。
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| 关 键 词： | 神经干细胞 神经巢蛋白 免疫磁珠法 |
| 文章编号： | 0258-879X(2000)11-1092-03 |
| 修稿时间： | 2000-06-28 |
Immunomagenetic indirect positive sorting of neural stem cells from fetal rat brain |
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| GAO Guo Yi ,LU Yi Cheng ,JIANG Ji Yao ,LI Li ,ZHANG Ling Zhen ,ZHU Cheng. Immunomagenetic indirect positive sorting of neural stem cells from fetal rat brain[J]. Former Academic Journal of Second Military Medical University, 2000, 21(11): 1092-1094 |
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| Authors: | GAO Guo Yi LU Yi Cheng JIANG Ji Yao LI Li ZHANG Ling Zhen ZHU Cheng |
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| Affiliation: | GAO Guo Yi 1,LU Yi Cheng 1,JIANG Ji Yao 1,LI Li 2,ZHANG Ling Zhen 2,ZHU Cheng 1 |
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| Abstract: | Objective: To study the sorting high purity neural stem cells from fetal rat brain cells. Methods: Fetal rat brain cell suspensions were incubated with monoclone antibody of nestin, and the labelled cells were separated from the suspension in the magnetic field by immunobeads coated with the second antibody. Purity of the sorted cells was determined with flow cytometry. Results: Purity of the sorted neural stem cells were determined as 93.0% 99.7%, active cells acount for 92% 97%. Conclusion: The magnetic cell sorting system can effectively separate neural stem cell from brain cell suspension. [ |
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| Keywords: | neural stem cell neural epithelial stem cell protein nestin magnetic beads |
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