益肾调经方对阿霉素肾病伴雷公藤多苷致性腺抑制的雌性大鼠肾保护作用的实验研究

目的：在阿霉素尾静脉注射造成微小病变肾病动物模型的基础上，建立雷公藤多苷致雌性肾病大鼠性腺抑制的模型，并观察益肾调经方（YSTJF）对其的影响，探讨YSTJF对阿霉素肾病伴性腺抑制的雌性大鼠肾保护作用。方法：雌性SD大鼠50只，随机挑选6只作正常对照组。余用阿霉素5mg／kg一次性尾静脉注射造成微小病变肾病动物模型，并随机分为6组：（1）肾病模型组：再予蒸馏水灌胃。（2）性腺抑制组：再予雷公藤多苷80mg·kg^-1·d^-1。（3）YSTJF高、中、低剂量组：予雷公藤多苷80mg·kg^-1·d^-1、YSTJF水煎浓缩液（分别为含生药75、50、25g·kg^-1·d^-1）合用每日灌胃。（4）西药对照组：予雷公藤多苷片80mg·kg^-1·d^-1和结合雌激素倍美力0．02mg·kg^-1·d^-1叫合用每日灌胃。该实验共8周，实验结束后收集标本，24h尿蛋白、N-乙酰-β-D-氨基葡萄糖苷酶（NAG），血肌酐（Scr），尿素氮（BUN）；光镜下观察肾脏组织的病理。结果：肾病模型组的尿蛋白、尿NAG酶明显高于正常对照组；性腺抑制组、YSTJF各剂量组和西药对照组能降低尿蛋白、尿NAG酶；、YSTJF中低剂量组降低尿蛋白与性腺抑制组比较（P〈0．05）；、YSTJF高剂量组降低尿NAG酶优于低剂量组和性腺抑制组。肾病模型组BUN高于正常对照组（P〈0．05）；性腺抑制组、、YSTJF各剂量组和西药对照组均低于肾病模型组（P〈0．05）；YSTJF各剂量组、西药对照组比性腺抑制组降低更明显（P〈0．05），YSTJF各剂量组比西药对照组为佳（P〈0．05）。从肾脏病理改变的小管间质病变积分分析：YSTJF各剂量组积分低于肾病模型组、性腺抑制组（P〈0．05），高、中剂量组较西药对照组为佳。结论：YSTJF可以通过降低尿蛋白、NAG酶、BUN，降低肾小管间质积分，改善肾小管间质病理改变，对于阿霉素肾病伴性腺抑制的雌性大

The Experiment Study of Yi Shen Tiao Jing Fang's Kidney Protection Effect on Female Hypogoandism Rats Induced by Tripterygium Wilfordii Ployglycosidium

Objective： To observe the protection effect of Yi Shen Tiao Jing Fang＇ s （YSTJF） kidney protection effect on female hytx：goandism rats induced by Tripterygium wilfordii ployglycosidium （TWP）. Methods： Among fifty female rats, 6 rats were randomly selected as normal control group,The others were induced minimal lesion nephropathy by Adriamycin（Adr） and divided into 6 groups randomly. （1）Adr nephropathy group： intragastric administrate with distilled water; （2）hytx：goandism group： intragastric administrate with TWP; （3）Three dosage of YSTJF groups： intragastric administrate with TWP and YSTJF（ low, medium and high doses） ; （4）Western medicine control group： intragastric administrate with TWP and Conjugated Estrogens（Premarin）. The research lasted for 8 weeks, then,24 - hour urinary protein, NAG, Scr, BUN were detected, renal pathomorphologic changes were examined under light microscope. Results：The level of urinary protein and NAG in Adr nephropathy group was significantly higher than in control group, lower in hypogoandism group, three YSTJF groups and Western medicine control group. Both urinary protein and NAG in YSTJF groups were lower than in hypogoandism group（ P 〈 0.05）. NAG was lower in high dose YSTJF group than in low dose group and hypogoandism group. BUN was higher in Adr nephropathy group than in normal control group（ P 〈 0.05）, lower in YSTJF groups and western medicine group than in hypogoandism group （P〈 0.05）, and more lower in YSTJF groups than in western medicine control group（ P 〈 0.05）. Scores of tubulointerstitial pathomorphologie changes of YSTJF groups were lower than Adr nephropathy groups and hypogoandism groups（ P〈O. 05）, besides, high and medium groups were lower than western medicine control groups. Conclusion： YSTJF not only decrease urireary protein, NAG, BUN, but also reduce scores of tubulointerstitial pathomorphologic changes, ameliorate tubulointerstitium, and it shows some kidney protection ef