| 纯化HBV促进人肝星状细胞系LX-2细胞Smad3表达 |
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| 引用本文： | 吴晓宁,白启轩,刘天会,李佳,尤红,贾继东. 纯化HBV促进人肝星状细胞系LX-2细胞Smad3表达[J]. 传染病信息, 2009, 22(4): 203-206 |
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| 作者姓名： | 吴晓宁 白启轩 刘天会 李佳 尤红 贾继东 |
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| 作者单位： | 首都医科大学附属北京友谊医院,北京,100050 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的体外实验观察纯化HBV对人肝星状细胞系LX-2细胞表达Smad3的影响。方法采用蔗糖密度梯度离心法从HBV感染者的高滴度血清中进行病毒纯化浓缩，然后应用MTT法观察纯化HBV对LX-2细胞增殖率的影响；再用纯化HBV培养LX-2细胞24h和48h后，应用实时荧光定量PCR方法检测细胞Smad3mRNA表达的变化，以Western blot方法检测细胞Smad3蛋白表达的变化。结果经蔗糖密度梯度离心可得到高纯度的HBV颗粒，10^3～10^7copies／ml的HBV可逐渐增加LX-2细胞的增殖率，纯化HBV培养LX-2细胞24h和48h后，Smad3mRNA表达分别为阴性对照的1．7倍和4．3倍。纯化HBV培养LX-2细胞24h后阴性对照组和纯化HBV组HSmad3／β-actin吸光度比值分别为0．62、0．91；培养48h后阴性对照组和纯化HBV组Smad3／-actin吸光度比值分别为0．33、1．02。结论纯化HBV可促进人肝星状细胞系LX-2细胞Smad3表达。
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| 关 键 词： | 乙型肝炎病毒 肝星状细胞 Smad3 |
Purified HBV promoting Smad3 expression in human hepatic stellate cell line LX-2 cells |
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| Wu Xiaoning,Bai Qixuan,Liu Tianhui,et al.. Purified HBV promoting Smad3 expression in human hepatic stellate cell line LX-2 cells[J]. Infectious Disease Information, 2009, 22(4): 203-206 |
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| Authors: | Wu Xiaoning Bai Qixuan Liu Tianhui et al. |
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| Affiliation: | Wu Xiaoning,Bai Qixuan,Liu Tianhui,et al.Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China |
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| Abstract: | Objective To evaluate the effect of purified hepatitis B virus(HBV) on Smad3 expression in human hepatic stellate cell(HSC) line LX-2 cells via in vitro study.Methods HBV of high-titer serum from HBV-infected patients was purified and concentrated by sucrose density gradient centrifugation.The effect of purified HBV on the proliferation rate of LX-2 cells was observed by MTT assays.After LX-2 cells were cultured with purified HBV for 24 and 48 hours,Smad3 mRNA expression was quantified by real-time fluoresc... |
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| Keywords: | Smad3 |
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