携Sialyl Lewis^X超声微泡靶向探查缺血心肌的炎症分子“印记”

目的探讨应用靶向超声分子成像探查心肌缺血再灌注损伤炎症“印记”的可行性。方法采用“亲和素-生物素”桥接法构建携唾液酸化路易斯（Sibyl Lewis^X）靶向超声微泡（MBsLex）和同型对照微泡（MBc）。10只心肌缺血再灌注小鼠随机先后注入MBsLex和MBc（间隔30min），分别于注入5min后行心肌对比超声检查，测量心肌缺血区和非缺血区的声强度（Ⅵ）。结果对比超声图像显示MBsLex组缺血区心肌造影见显著增强，Ⅵ值高达23．52±1．08，而在MBc组缺血区心肌造影仅见轻度增强，Ⅵ缸为9．81±0．41，两者之间差异有统计学意义（P〈0．05）。但无论MBsLex组还是MBc组，缺血区心肌Ⅵ值均明显高于非缺血区心肌Ⅵ值（P〈0．05）。两组非缺血区心肌之间Ⅵ值未见明显差异。结论应用携sLex超声微泡行对比超声能够靶向探查心肌缺血再灌注损伤的炎症“印记”。

Targeted detection of inflammatory 'imprint' in ischemic myocardium by ultrasound microbubbles with sialyl lewisX

Objective To explore the feasibility of inflammatory ＂imprint＂ in myocardial ischemical reperfusion injury detected by targeted myocardial contrast eehocardiography （MCE）. Methods Targeted ultrasound microbubbles with sialyl Lewisx （sialyl Lewis^x, sLex） and isotype control microbubbles were conjugated by ＂avidin- biotin＂ bridge. 10 mice with myocardial ischemical repeffusion were injected with MBsLex and MBc randomly （ interval was 30 rain ）, then examined by myocardial contrast echocardiography （MCE） after 5 rain, the video intensity （Ⅵ） was determined. Results Contrast eehocardiography images showed ischemic region myocardium was enhanced obviously in MBsLex group, Ⅵ was （23.52 ± 1.08）, and iscbemic region myocardium was enhanced in group MBc with VI of （9.81 ± 0.41 ）, there was significant difference between the two groups（ P 〈 0.05）. The Ⅵ of ischemic region myocardium was obviously higher than that in non - isehemic myocardium （ P 〈 0.05 ）. There was no significant difference in Ⅵ between the two groups. Conclusion Molecular imaging of seleetin with targeted contrast echoeardiography is possible to detect myocardial ischemiareperfusion injury effectively.