梗阻性黄疸大鼠胆总管再通对丙泊酚麻醉敏感性的影响

目的探讨梗阻性黄疸大鼠胆总管再通后对丙泊酚麻醉敏感性的影响。方法清洁级成年雄性SD大鼠24只,8周龄,体重200~300g,采用随机数字表法分为三组:假手术组(S组)、普通黄疸组(I组)和可逆性黄疸组(R组)。分别在术前及术后3、7、14、21d通过尾静脉采集大鼠血液标本检测血清总胆红素(TBL)和总胆汁酸(TBA)浓度。分别在胆总管结扎前和结扎后7、21d测定大鼠的翻正反射消失时间和恢复时间。结果术后3、7、14、21dI组血清TBL和TBA浓度,R组血清TBA浓度明显高于S组(P0.05);术后3、7、14dR组血清TBL浓度明显高于S组(P0.05);术后14、21dR组大鼠血清TBL和TBA浓度明显低于I组(P0.05)。术后7dI组和R组的翻正反射消失时间明显短于,恢复时间明显长于S组(P0.05)。结论梗阻性黄疸时大鼠对丙泊酚的麻醉敏感性增加,胆管再通后增加的麻醉敏感性会逐渐恢复。

Effect of anesthetic sensitivity to propofol after biliary decompression in common bile duct ligation rats

Objective To investigate if anesthetic sensitivity to propofol will be restored after biliary decompression. Methods Twenty-four adult male SD rats were randomly assigned into 3 groups: sham group (group S), irreversible obstructive jaundice group (group I) and reversible obstructive jaundice group (group R). The serum total bilirubin (TBL) and total bile acid (TBA) concentratins were detected in the rat blood samples collected from the caudal vein before and after the operation, 3, 7, 14, 21 d respectively. Propofol was administered to measure the time of loss of righting reflex and recovery pre or 7th and 21th day post ligation. Results Serum TBL and TBA in group I and serum TBA in group R were significantly higher than that in group S on 3rd, 7th, 14th, 21th day post-surgery(P<0.05). Compared with group S, seum TBL in group R were significantly high on 3rd, 7th, 14th day post-surgery. Serm TBL and TBA in group R were significantly lower than group I on 14th, 21th day post-surgery (P<0.05). Compared with group S, the time to loss of righting reflex in group I and group R were significantly shortened and the time to recovery were significantly increased on 7th day post-surgery (P<0.05). Conclusion Obstructive jaundice could significantly potentiate the ability of propofol to induce a loss of righting reflex, and the increased anesthesia sensitivity will be restored after biliary decompression.