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Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples
Authors:Bracca Andrea  Tosello María Elena  Girardini Javier E  Amigot Susana L  Gomez Carlos  Serra Esteban
Affiliation:Instituto de Biología Molecular y Celular de Rosario, IBR-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, 2000 Rosario, Argentina.
Abstract:We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.
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