Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples |
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| Authors: | Bracca Andrea Tosello María Elena Girardini Javier E Amigot Susana L Gomez Carlos Serra Esteban |
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| Institution: | Instituto de Biología Molecular y Celular de Rosario, IBR-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, 2000 Rosario, Argentina. |
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| Abstract: | We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens. |
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