腺苷预处理培养大鼠心肌细胞后酶活性变化和超微结构观察

目的 :应用组化方法和透射电镜 ,观察腺苷预处理培养大鼠心肌细胞琥珀酸脱氢酶、细胞色素氧化酶及Ca2 +,Mg2 +依赖性ATP酶活性细胞超微结构的变化。方法 :取SD大鼠乳鼠心室肌细胞在无菌状态下 ,用DMEM常规培养 5天后分四组 :正常对照组、拟缺血再灌注组、拟缺血预处理组、腺苷预处理组 ,用组化方法检测心肌酶活性 ;以透射电镜观察细胞形态结构变化。结果 :在缺血预处理组和腺苷预处理组琥珀酸脱氢酶、细胞色素氧化酶、Ca2 +,Mg2 +依赖性ATP酶活性均高于拟缺血再灌注组 ,且细胞结构保存良好 ;缺血预处理组、腺苷预处理组与正常对照组无显著性差异。结论 :腺苷预处理对缺血再灌注心肌细胞具有类似缺血预处理的保护作用

CHANGES IN ENZYMATIC ACTIVITY AND OBSERVATION OF CULTURED CARDIAC MYOCYTES OF ADENOSINE-PRECONDITIONED RATS

Objective:To study the activities of Succinate Dehydrogenase,Cytochrome Oxidase Ca 2+ ,Mg 2+ ATP ase and the ultrastructure in cultured cardial myocytes in rats preconditioned with adenosine.Methods:The cardiac myocytes of neonatal rats were cultured.The cardiac myocytes were divided into 4 groups:normal control group,ischemia/reperfusion group,ischemic preconditioned group and adenosine preconditioned group aftre 5 d culture.Activities of cardiac myocytes enzymes were tested by histochemistry and ultrastructure was observed by electronic microscope.Results:The activities of Succinate Dehydrogenase,Cytochrome Oxidase,Ca 2+ ,Mg 2+ ATPase in ischemic preconditioned group,adenosine preconditioned group were significantly higher than that in ischemia/reperfusion group and the ultrastructure of cardiac myocyte in ischemic preconditioned group,adenosine preconditioned group were maintained well.Ischemic precondition group and adenosine precondition group were not lower than control group.Conclusion:It is suggested that adenosine has protective effects on ischemia/reperfusion of cultured cardiac myocytes,which is similar to that of ischemic preconditioned.