Activation of the 2′-N-Acetyltransferase Gene [aac(2′)-Ia] in Providencia stuartii by an Interaction of AarP with the Promoter Region |
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| Authors: | David R. Macinga Michael R. Paradise Milica M. Parojcic Philip N. Rather |
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| Affiliation: | Departments of Medicine and of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA. |
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| Abstract: | The aac(2')-Ia gene in Providencia stuartii encodes a 2'-N-acetyltransferase capable of acetylating both peptidoglycan and certain aminoglycoside antibiotics. Regulation of the aac(2')-Ia gene is influenced in a positive manner by the product of the aarP gene, which encodes a small transcriptional activator of the AraC (XylS) family. In this study, we demonstrate the sequence requirements at the aac(2')-Ia promoter for AarP binding and activation. |
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