| 强直性脊柱炎患者外周血单个核细胞HLA-B27抗原的纯化 |
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| 引用本文： | 官健,金大地,史占军. 强直性脊柱炎患者外周血单个核细胞HLA-B27抗原的纯化[J]. 中华风湿病学杂志, 2000, 4(4): 216-217 |
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| 作者姓名： | 官健 金大地 史占军 |
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| 作者单位： | 第一军医大学南方医院脊柱骨病科(官健!510515广州,金大地!510515广州,史占军!510515广州),第一军医大学南方医院外科实验室(丁红!510515广州,陆洁!510515广州,曹琼!510515广州,武大林!510515广州) |
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| 摘 要： | 目的 纯化强直性脊柱炎患者外周血单个核细胞表面的HLA B2 7抗原。方法 HLA B2 7单抗 (FD70 5 )亲和层析法。结果 纯化的抗原蛋白经SDS PAGE显示为单一条带 ,经分析其相对分子量为 5 6 40 0。结论 经此法可成功纯化强直性脊柱炎患者外周血单个核细胞HLA B2 7抗原。
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| 关 键 词： | 脊柱炎 强直性 色谱法 亲和 HLA-B27抗原 |
| 修稿时间： | 1999-11-12 |
Purification of HLA-B27 on peripheral blood mononuclear cells from patients with ankylosing spondylitis |
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| Abstract: | Objective To purify HLA B27 on the surface of peripheral blood mononuclear cells from patients with ankylosing spondylitis.Method Specific affinity column,cyanogen bromide activated sepharose 4B column coupled to anti HLA B27 monoclonal antibodies,was used for HLA B27 purification.Results The purified antigen by this method showed a single band by SDS PAGE,with a molecular mass of 56 400.Conclusion HLA B27 on the surface of peripheral blood mononuclear cells from patients with ankylosing spondylitis is successfully purified. |
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| Keywords: | Spondylitis ankylosing Chromatography affinity HLA-B27 antigen |
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