醛固酮刺激心肌成纤维细胞增殖的实验研究

目的探讨醛固酮在致心肌纤维化中的作用。方法以培养的FBs为模型，观察醛固酮刺激FBs增殖，螺内酯阻断醛固酮的作用，用^3H-亮氨酸及^3-胸腺嘧啶掺入量作为反应FBs增殖的指标。结果醛固酮呈浓度依赖性的促FBs蛋白核酸合成速率明显增高，与对照组相比差异显著（P〈0．01）；螺内酯能明显抑制醛固酮介导的FBs蛋白核酸合成速率增高，与醛固酮刺激组相比差异显著（P〈0．01）。结论螺内酯抑制醛固酮刺激的FBs增殖。

Rat Cardiac Fibroblasts Proliferation by Aldosterone

Objective To investigate the effect of aldosterone on proliferation of rat cardiac fibroblasts.Methods Upon the model of cultured rat FBs,aldosterone was used to stimulate proliferation of FBs,()~3H-Leucine(()~3H-Leu) and()~3H-Thymidine(()~3H-TdR) incorporation as the target to evaluate FBs proliferation.Results Synthesis rates of protein and nucleic acid of FBs stimulated by aldosterone increased in a dose dependent manner significantly in contrast to control(P<0.01);spironoactone markedly inhibited syntheses of protein and nucleic acid mediated by aldosterone in FBs with a significant difference from aldosterone-stimulated group(P<0.01).Conclusions Spironoactone inhibits the proliferation of FBs induced by aldosterone.