组织工程角膜支架材料再生丝素膜的生物相容性

目的:研究再生丝素膜作为组织工程角膜支架材料的可行性。方法:制备再生丝素膜,测定其接触角与透光率;在材料上接种兔角膜上皮细胞,观察细胞生长情况,MTT法测定6h贴壁率;将膜材料植入兔角膜基质层间,观察材料的透明性、可降解性及生物反应性。结果:再生丝素膜材料接触角为58.2度;492nm波长透光率为93.0%,570nm波长透光率为94.4%;细胞在材料表面贴附良好,6h贴壁率为31.6%,经统计学比较优于普通培养板;4wk时材料开始变得不透明,从角膜缘长入新生血管,8wk时新生血管达到高峰,12wk时材料部分降解,至16wk材料大部分降解,新生血管显著减少。结论:再生丝素膜具有较好的生物相容性。

Biocompatibility of silk fibroin membrane as tissue engineering corneal scaffold

AIM:To investigate the feasibility of using silk fibroin membrane as tissue engineering corneal scaffold.METHODS:Silk fibroin membrane was prepared.Contact angle survey and transmittance measure were conducted.Cornea epithelial cell of rabbits was cultured on the membrane and the cell growth was observed.MTT assay was used to detect the 6-hour cell adhesion rates.Then,the material was implanted into the stroma of the rabbits and its transparency,degradability and biocompatibility.were evaluated.RESULTS:The contact angle of silk fibroin membrane was 58.2 degree.The transmittance was 93.0% under 492nm and 94.4% under 570nm.The 6-hour cell adhesion rate was 31.6%,compared with normal culture plate by statistic.The membrane was translucent 4 weeks after implantation,meanwhile,neovascularization was obse-rved in limbus.New vessels grew to the peak after 8 weeks.The material was partly degraded at 12th week.Few opaque pieces and vessels remained in the cornea after 16 weeks.CONCLUSION:Silk fibroin membrane has good biocompatibility and can become a new choice of tissue engineering corneal scaffold.