食管鳞癌组织中RhoC和Kiss-1 mRNA的表达及意义*

目的:探讨食管鳞状上皮细胞癌组织、正常食管黏膜组织中RhoC mRNA和Kiss-1 mRNA 的表达及其与临床病理因素的关系。方法:收集河南省安阳市肿瘤医院手术切除食管癌标本62例及正常食管黏膜组织62例；应用原位杂交法检测每例食管组织中RhoC mRNA、Kiss-1 mRNA 的表达情况。采用SPSS13.0 软件行χ2检验及Spearman 相关分析进行相关统计。结果:RhoCmRNA 在正常食管黏膜、食管鳞癌组织的阳性表达率分别为21.0% 、80.6% ，组间比较差异有统计学意义（χ2=44.173，P<0.05），在不同分化程度组、不同浸润深度组及有无淋巴结转移组的食管鳞癌组织中RhoC mRNA的阳性表达率差异均有统计学意义（χ2分别
为9.520、4.478、3.898，P 均<0.05）；食管鳞癌组织中Kiss-1 mRNA 的阳性表达显著低于正常食管黏膜Kiss-1 mRNA 的阳性表达，表达率分别为51.6% 95.2%（χ2=30.102，P<0.05），有淋巴结转移组及无淋巴结转移组的食管鳞癌组织中Kiss-1 mRNA 的阳性表达率分别为30.0% 、61.9% ，二者相比差异有统计学意义（χ2=5.522，P<0.05），在不同分化程度组、不同浸润深度组食管鳞癌组织的Kiss-1 mRNA 阳性表达率差异无统计学意义（χ2分别为0.758、1.241，P 均>0.05）；食管鳞癌组织中RhoC与Kiss-1 mRNA 的表达呈负相关关系（γ =-0.311，P<0.05）。 结论:RhoC mRNA高表达和Kiss-1 mRNA 表达的降低或缺失可能在食管鳞癌的发生、发展过程中起重要作用。联合检测二者可望作为判断食管鳞癌侵袭转移能力的一项客观指标。 

The Expression and Significance of RhoC and Kiss-1 mRNA in Esophageal Squamous Cell Carcinoma

Objective:To investigate the expression of RhoC mRNA and Kiss- 1 mRNA expression in esophageal squa-mous cell carcinoma (ESCC) and normal esophageal epithelia.Methods:We collected 62samples of ESCC tissue and 62 samples of normal esophageal epithelia in Anyang Cancer Hospital. The expression of RhoC mRNA and Kiss- 1 mRNA was detected with in situ hybridization. Chi-square test and Spearman correlation analysis were used to analyze the results.Re? sults: The expression levels of RhoC mRNA in ESCC tissues and normal esophageal epithelia were21.0% and 80.6%, re-spectively, with a significant difference ( χ2=44.173 , P<0.05). For ESCC, the expression level of RhoC mRNA was correlated with the degree of differentiation, depth of invasion and lymph node metastasis ( P<0.05). The positive rate of Kiss-1 mRNA expression in cancer tissues was lower than that in normal mucosa, with a significant difference ( χ2=30.102 , P<0.05). The positive rates of expression of Kiss-1 mRNA in the group with lymph node metastasis was 30.0%, while in the group with-out lymph node metastasis, it was 61.9%, with a significant difference between them (P<0.05). For ESCC, the expression level of Kiss- 1 mRNA was not correlated with the degree of differentiation and depth of invasion (P>0.05) There was a sig -nificant negative correlation between RhoC and Kiss-1 (P<0.05). Conclusion:The increased expression of RhoC mRNA and loss or decreased expression of Kiss-1 mRNA play an important role in carcinogenesis, invasion, and metastasis of esophageal squamous cell carcinoma. Detection of RhoC and Kiss-1 expression can evaluate potential lymph node metas-tasis of esophageal squamous cell carcinoma.