万古霉素发酵液凝聚与絮凝研究

对凝聚与絮凝法预处理万古霉素发酵液的工艺进行了研究，初步选用三种凝聚剂、三种絮凝剂，以滤速、沉淀物重量、滤液澄清度、滤液中万古霉素含量以及万古霉素液相色谱峰面积占总积分面积百分比为指标，考察凝聚剂与絮凝剂的作用效果。从中选择偏铝酸钠、壳聚糖，并详细研究其用量、pH对预处理效果的影响，结果显示偏铝酸钠为较合适的凝聚剂。进一步讨论了搅拌时间对凝聚效果的影响，通过正交试验确定了最佳凝聚工艺条件为：pH4，偏铝酸钠用量750mg／L，搅拌时间6min。

Study on coagulation and flocculation of vancomycin fermentation broth

Coagulation and flocculation process of vancomycin fermentation broth were studied.Three coagulants and three flocculants were chosen to examine their effects on the pretreatment process.The factors including filtration rate,precipitation weight,filtrate clarifying level and vancomycin concentration in the filtrate were considered.Sodium meta-aluminate and chitosan were selected.The effects on the pretreatment by coagulant/flocculant concentration and pH value were studied in detail.Sodium meta-aluminate was considered to be a suitable coagulant.Further experiments were carried out to study how the stirring time effected the coagulation.The optimal coagulation process condition was obtained by orthogonal design experiment and the parameters were determined as follows: pH4;concentration of sodium meta-aluminate,750 mg/L;stirring time: 6 min.