大蒜素对大鼠心室肌细胞L-型钙通道的影响

目的:探讨大蒜素对大鼠心室肌细胞L-型钙通道的作用.方法:用急性酶解法获得大鼠的单个心肌细胞,用标准的全细胞膜片钳技术记录钙通道电流.结果:5、50、250和500μmol/L大蒜素分别抑制钙电流4.4%、26.91%、38.06%、72.90%.250μmol/L大蒜素能使心肌细胞钙电流-电压曲线明显上移,峰电流密度从(7.17±0.65)pA/pF减少至(4.44±0.52)pA/pF(n=15,P<0.05),但激活电位、峰电位和翻转电位无明显改变(P>0.05).大蒜素能使钙电流失活曲线明显左移(P<0.05).大蒜素对钙电流激活曲线、失活再复活曲线和频率依赖性无明显影响(P>0.05).结论:大蒜素呈浓度依赖性地抑制心肌细胞L-型钙通道.

Effect of allitridi on calcium current in rat ventricular myocytes

Objective: To determine the effect and mechanism of allitridi on L-type calcium current (ICa,L). Methods: Whole-cell pacth clamp recording technique was used to record ICa, L in single cells isolated rat ventricular myocytes. Results: The nifedipine-sensitive L-type calcium current was recorded in the rat ventricular myocytes. Allitridi decreased the calcium channel current in a dose-dependent and voltage-dependent manner in ventricular myocytes of rats. 1. The current-voltage curve was shifted upwards, which active potential,peak potential and reverse potential had no significant changes. 2. The inactivation curve was shifted to more negative potential, but the activation curve and the recovery curve were not altered. 3. Allitridi had no effect on frequent-dependency of calcium channel. Conclusion: Allitridi concentration- dependently decreased calcium channel current in ventricular myocytes of rat.