| 多重PCR法检测Y染色体微缺失 |
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| 引用本文： | 林美珊,郑宇琼,张伶俐,陈林兴,林广玲.多重PCR法检测Y染色体微缺失[J].江西医学检验,2007,25(2):111-112,188. |
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| 作者姓名： | 林美珊 郑宇琼 张伶俐 陈林兴 林广玲 |
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| 作者单位： | 广东省汕头大学医学院第二附属医院，汕头大学医学院第一附属医院，广东省汕头大学医学院第二附属医院，广东省汕头大学医学院第二附属医院，广东省汕头大学医学院第二附属医院 |
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| 摘 要： | 目的探讨多重PCR法检测Y染色体微缺失对男性不育患者的病因学诊断价值。方法采用多重PCR法检测男性不育患者和正常男性Y染色体AZF座位的缺失情况。结果106例患者中Y染色体微缺失28例,缺失率26.4%,30例正常男性Y基因均未发现缺失。结论Y染色体微缺失是不明原因性无精子症或严重少精子症的病因之一,多重PCR检测技术具有快速、高效、灵敏度高等特点。
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| 关 键 词： | 多重PCR Y染色体微缺失 无精子症 少精子症 男性不育 |
| 文章编号： | 1008-0023（2007）02-0111-03 |
Application on Y chromosmal microdeletions with gene chip technique in male infertility |
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| LIN Meishan, ZHENG Yuqiong, ZHANG Lingli,et al..Application on Y chromosmal microdeletions with gene chip technique in male infertility[J].Jiangxi Journal of Medical Laboratory Sciences,2007,25(2):111-112,188. |
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| Authors: | LIN Meishan ZHENG Yuqiong ZHANG Lingli |
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| Institution: | The Second Affiliated Hospital, Shantou University Medical College. Gucmgdong Shantou 515041, China |
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| Abstract: | Objective To study the diagnosis value on Y chromosmal microdeletions with gene chip technique in male infertility.Methods Y chromosmal microdeletions were examined from 106 male patients with azoospermia or oligospermia and 30 controls by gene chip technique.Results Among 106 patients,the ratio of Y chromosmalemicrodeletions was 26.4%(28/106),and no positive ones was found in the controls.Conclusions Y chromosmal microdeletions can cause male azoospermia or ologosepermatism.The gene chip technique is a quick,effective,sensitive method for detecting Y chromosmal microdeletions. |
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| Keywords: | Male infertility Y chromosomal microdeletions Azoospermia Oligosepermatism Multiplex PCR |
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