准分子激光屈光性角膜切削术后角膜上皮素mRNA的表达

目的：观察准分子激光屈光性角膜切削术（photorefractive keratectomy,PRK)术后,新西兰大白兔角膜组织愈合过程中角膜上皮素（keratoepithelin,KE)mRNA表达的变化,探讨KE与角膜雾状混浊（haze）的关系。方法：24只新西兰大白兔中的20只左眼按－10D行PRK术（另外4只为正常对照组）,分别于术后7、14、28天、3个月和6个月行光镜、电镜和原位杂交检查。结果：正常角膜上皮和基质中未检测出KE mRNA;术后7、14、28天角膜上皮KE,mRNA表达阳性,术后3月和6月阴性;术后7天和14天基质中KE mRNA表达阳性,术后28天、3月和6月表达阴性。结论：角膜上皮素参与了PRK术后伤口愈合中细胞外基质的形成,可能在角膜组织的重建中起着重要的作用。

mRNA Expression of Keratoepithelin in Cornea after Photorefractive Keratectomy in Rabbits

Objective: To investigate the roles of keratoepithelin(KE)during healing after photorefrac-tive keratectomy (PRK).Methods: 20 of 24 rabbits underwent bilateral 193 nm excimer laser photorefractive keratectomy to correct 10 diopters of myopia, and the other 4 rabbits as normal group. The mRNA levels of KE in corneas of the rabbits were determined by the in situ hybridization technique with a Digoxigenin labled probe.Results: In normal group, there was no mRNA expression of KE in cornea, on 7, 14 days after operation, mRNA expression of KE was observed in epithelium and stroma of operation group, while on 28 days mRNA expression of KE was observed only in epithelium.Conclusion: KE was involved in the synthesis of extracellular matrix during healing after PRK and may play an important role in the development of haze by maintaining proper spacing among collagen fibrils. Eye Science 2000; 16: 235-238.