苋科植物雁来红叶提取物对大鼠的胃细胞保护及抗分泌作用

目的：验证苋科植物雁来红（Amaranthustricolor Linn.）叶的提取物对不同胃溃疡模型大鼠的抗溃疡作用。方法：通过5种不同的大鼠胃溃疡模型（乙酸、幽门结扎、乙醇、消炎痛及缺血再灌注模型）证实雁来红叶对大鼠胃分泌功能的影响及胃细胞的保护作用。不同的雁来红叶的提取物（乙醇、石油醚、三氯甲烷及乙酸乙酯）以200mg/kg的剂量给予大鼠服用以检测其功效。结果：急性口服毒性实验显示各种提取物的安全口服剂量可达2000mg/kg,故选取该剂量的十分之一即200mg/kg作为实验用剂量。乙醇提取物及乙酸乙酯提取物对乙酸所致大鼠慢性胃溃疡有治愈作用;对幽门结扎大鼠有抑制其胃分泌功能的作用;对乙醇及消炎痛所致胃溃疡大鼠有胃细胞保护作用。而石油醚及三氯甲烷提取物没有明显的抗大鼠胃溃疡的作用。结论：本研究证明雁来红叶的提取物对实验性大鼠胃溃疡有很好的治疗作用,这一作用与文献所报道的该植物在民间医学中的应用相符。

Gastric antisecretory and cytoprotective effects of leaf extracts of Amaranthus tricolor Linn. in rats

Objective:The present study was aimed to evaluate the antiulcer activity of leaf extracts of Amaranthus tricolor Linn.(Amaranthaceae) in rats.Methods:The effects of A.tricolor leaves on gastric secretion and the effect of gastric cytoprotection were evaluated using five different models of gastric ulcers:acetic acid-induced,pylorus ligation-induced,ethanol-induced,indomethacin-induced and ischemia-reperfusion-induced gastric ulcers.The different extracts,namely,ethanolic extract (EAT),petroleum ether extract (PEAT),chloroform extract (CAT) and ethyl acetate extract (EAAT) of A.tricolor leaves were administered at a dose of 200 mg/kg per oral (p.o.).Results:The acute oral toxicity study revealed that all the extracts were safe up to 2 000 mg/kg,p.o; hence one-tenth of this dose was selected for evaluation of antiulcer activity.The EAT and EAAT (200 mg/kg,p.o.) showed gastric ulcer-healing effect in acetic acid-induced chronic gastric ulcers.The EAT and EAAT inhibited gastric secretion in pylorus-ligated rats and showed gastric cytoprotective effect in ethanol-induced and indomethacin-induced gastric ulcers,while PEAT and CAT showed no significant antiulcer effect.Conclusion:The leaf extracts of A.tricolor are found to possess very good antiulcer property in the experimental animal models of gastric ulcers which is consistent with the literature report in folk medicine.