突厥蔷薇提取物抗戊四唑所致小鼠痉挛的作用

目的：研究突厥蔷薇（Rosa damascena）的水提物、乙醇提取物及三氯甲烷提取物对戊四唑所致小鼠惊厥的作用。方法：实验动物按如下方案分组：生理盐水对照组,地西泮组（3mg/kg）,水提物组（100,500和1000mg/kg）,乙醇提取物组（100,500和1000mg/kg）,三氯甲烷提取物组（100,500和1000mg/kg）。所有药物均在注射戊四唑30min前皮下注射。记录并比较各组小鼠阵发性强直性抽搐潜伏期、最小阵发性痉挛潜伏期及死亡率。结果：与生理盐水对照组相比,三组水提物组小鼠的阵发性强直性抽搐潜伏期及最小阵发性痉挛潜伏期均有延长（P〈0.05或P〈0.01）;一组乙醇提取物组（1000mg/kg）小鼠的最小阵发性痉挛潜伏期及两组乙醇提取物组（500和1000mg/kg）小鼠的阵发性强直性抽搐潜伏期均有延长（P〈0.05,P〈0.01）;三组三氯甲烷提取物组小鼠的阵发性强直性抽搐潜伏期及最小阵发性痉挛潜伏期无明显变化。不同提取物各组的死亡率与对照组相比无明显差异。结论：本研究的结果证实了突厥蔷薇具有抗戊四唑所致小鼠惊厥的作用,其具体作用机制有待进一步研究。

Effects of different extracts of Rosa damascena on pentylenetetrazol-induced seizures in mice

In the present study,the effects of aqueous,ethanolic and chloroformic extracts of Rosa damascena on pentylenetetrazol (PTZ)-induced seizures were investigated in mice.Methods:The animals were divided into the following groups:normal saline control group,diazepam group (3 mg/kg),three aqueous extract groups (100,500 and 1 000 mg/kg),three ethanolic extract groups (100,500 and 1 000 mg/kg) and three chloroformic extract groups (100,500 and 1 000 mg/kg).The extracts,normal saline or diazepam were injected intraperitoneally 30 min before PTZ injection.Latency to the first minimal clonic seizure (MCS) and generalized tonic-clonic seizure (GTCS) and the percent of mortality of rats in each group were recorded.Results:Significant increases in both MCS and GTCS latencies were observed in all the three aqueous extract groups in comparison with the normal saline control group ( P＜0.05 or P＜0.01).The MCS latency in the ethanolic extract (1 000 mg/kg) group and the GTCS latencies in the two ethanolic extract (500 and 1 000 mg/kg) groups were higher than those in the normal saline control group (P＜0.05,P＜0.01).There were no significant differences in MCS and GTCS latencies between the three chloroformic extract groups and the normal saline control group.No significant differences were seen in mortality rate following PTZ administration between the different extracts-treated mice and the control mice.Conclusion:The results of the present study showed that R.damascena has an anticonvulsant effect in a mouse model of PTZ-induced seizures but the exact mechanism of this effect should be clarified in future studies.