酒客乐对大鼠酒精性肝纤维化疗效及机制的研究

目的：探讨中药复方酒客乐对大鼠酒精性肝纤维化的防治作用及其机制。方法：采用白酒-吡唑-橄榄油的混悬液灌胃建立酒精性肝纤维化大鼠模型。雄性Wistar大鼠随机分为正常对照组、造模组、治疗组及酒客乐高、低剂量预防组。实验第12周后,除治疗组外,采集标本;治疗组于实验第16周后采集标本,观察酒客乐对大鼠体重、肝功能、血清PⅢP、LN含量以及肝脏病理形态改变、肝脏表达TGF-β1水平的影响。结果：造模组大鼠体重、血清ALT、AST、PⅢP、LN、肝脏表达TGF-β1水平、肝脏病理形态改变与正常对照组比较差异有显著性意义（P〈0.01）。酒客乐高剂量预防组大鼠体重增长与正常对照组差异无显著性意义（P〉0.05）。酒客乐高、低剂量预防组、治疗组大鼠血清ALT、AST、PⅢP、LN含量明显低于造模组（P〈0.01）。酒客乐能减轻大鼠肝脏病理形态改变,抑制其肝脏TGF-β1产生,酒客乐高、低剂量预防组、治疗组大鼠TGF-β1水平含量明显低于造模组,差异有显著性意义（P〈0.01）。结论：酒客乐具有防治大鼠酒精性肝纤维化的作用,其作用机制可能为：①直接作用：抑制肝脏细胞外基质的沉积,减轻肝脏病理形态改变;②间接作用：保护肝功能,增强免疫功能,纠正肝脏代谢紊乱。

Compound formula Jiukele's efficacy and its mechanisms in prevention and treatment of experimental alcoholic liver fibrosis in rats

Objective：To investigate the efficacy of Chinese compound formula Jiukele in the prevention and treatment of ethanol-induced liver fibrosis in the rats and its mechanisms.Methods：Forty male Wistar rats were divided randomly into 5 groups： normal control group,model group,therapy group,high and low dose prophylactic group.The mixture of alcohol（55.）,pyrazole and olive oils was fed to the stomach of the rats of model group every morning（7∶ 00-8∶ 00）.The rat model was successfully established in 12 weeks.The Jiukele was given to the rats of therapy group for another 4 weeks after rat model was established successfully.In high and low dose prophylactic group,the mixture was fed to the rats every morning and Jiukele was fed to the rats after 2 hours.The experiment was over in twelve weeks,except the therapy group,blood samples and liver tissues were collected.The samples of therapy group were collected after additional 4 weeks.The weight of the rats,liver function,the content of serum PⅢP,LN,the morphology and the lever of TGF-β1 in liver tissure were observed.Results：In model group,the content of serum ALT,AST,PⅢP,LN,the weight of rats,the pathology morphology of liver tissures,the expression lever of TGF-β1 in the liver tissure were significantly higher than normal control group（P0.01）.The weight of the rats in high dose prophylactic group was almostly increased the same as the normal control group（P0.05）.In high and low dose prophylactic group and the therapy group,the content of the serum ALT,AST,PⅢP,LN were obviously lower than the model group（P0.01）.The contents of TGF-β1 in the liver tissures of model group was greatly higher than other groups（P0.01）,the pathology morphology change was also more serious in liver tissues of model group than other groups.Conclusion：Jiukele has effects in prophylaxis and the treatment of alcoholic liver fibrosis in rats.The mechanisms may be as follows：to suppress the deposition and accelerate degradation of liver extracellular matrix,alleviate the pathology morphology change of liver tissure;to improve liver function,immune function,adjust liver metabolism disorder.