攻毒扶正法对非酒精性脂肪性肝炎大鼠瘦素、胰岛素的影响

目的：观察攻毒扶正法对非酒精性脂肪性肝炎（NASH）大鼠瘦素（LEP）、胰岛素（INS）的影响。方法：用高脂饲料与四氯化碳（CCl4）制备NASH大鼠模型,分别用乙肝系列中成药（乙肝清热解毒颗粒、乙肝益气解郁颗粒、乙肝养阴活血颗粒）、辛伐他丁溶液进行灌胃。分为正常组,模型组,辛伐他丁组,清热解毒组,益气解郁组,养阴活血组,清热解毒合益气解郁组（简称清解合益气组）,清热解毒合养阴活血组（简称清解合养阴组）共8组,每组12只。用药4周后显微镜下观察NASH大鼠肝脏HE染色切片,并对肝组织病变程度评分。检测肝功能、血脂、LEP、INS情况。结果：模型组肝组织病变程度评分明显高于正常组（P〈0.01）,丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）升高,总胆固醇（TCh）升高明显（P〈0.01）,LEP、INS水平均升高（P〈0.05）。治疗组均可降低LEP水平,肝组织病变评分降低。中药组的ALT、AST、TC、LEP、INS含量有下降趋势（P〈0.05或〈0.01）;辛伐他丁组能降低ALT、INS含量,差异无显著性意义。结论：NASH大鼠体内有明显LEP、INS水平升高趋势。各治疗组均能降低大鼠体内LEP、INS水平。通过各类指标的检测,中药组改善作用更为明显。证明攻毒扶正法治疗NASH大鼠有切实的作用。

Effect of Gongdu Fuzheng therapy on leptin and insulin in rats with nonalcoholic steatohepatitis

Objective: To observe the effect of Gongdu Fuzheng therapy(GFT) on leptin and insulin in rats with nonalcoholic steatohepatitis(NASH) Methods:Experimental animal models of NASH were established,treated with Qingrejiedu granules,Yiqijieyu granules,Yangyinhuoxue granules,simvastatin solution.All rats wrere divided into eight groups(n=12).After 4w,their AST,ALT,TC,leptin,insulin and HE change.were observed.Results:Model group,the liver lesion scores were significantly higher than the normal group(P<0.01),and A...