柴胡疏肝散及其拆方对抑郁模型大鼠行为及海马、杏仁核、额叶BDNF及其受体TrkB的影响

目的 探讨柴胡疏肝散对抑郁模型大鼠行为学及海马、杏仁核、额叶皮质脑源性神经营养因子（brain derived neurophic factor, BDNF)及其受体酪氨酸激酶受体B（TrkB）表达的影响。

Effects of Chaihu Shugan Powder on the Behavior and Expressions of BDNF and TrkB in the Hippocampus,Amygdala,and the Frontal Lobe in Rat Model of Depression

Objective To investigate the effects of Chaihu Shugan Powder（CHSGP） on the behavior and the expressions of brain-derived neurophic factor（BDNF） and its receptor tyrosine kinase receptors B（TrkB） in the hippocampus,amygdala,and the frontal lobe of depression model rats.Methods Sixty adult Sprague-Dawley rats were randomly divided into 6 groups,i.e.,the normal control group（NC）,the model control group（MC）,the CHSGP group,the disassembly 1 group（CⅠ）,the disassembly 2 group（CⅡ）,and the Fluoxetine control group（FC）,10 in each group.Except those in the NC,the rest rats were singly housed and exposed on an unpredicted sequence of mild stressor.From the fifteenth day,all rats were administered with equal volume of normal saline（to the NC group and the MC group） and of corresponding medicinal liquid（5.9 g/kg to the CHSGP group,3.3 g/kg to the CⅠ group,2.6 g/kg to the CⅡ group,and 1.8 mg/kg to the FC group） by gastrogavage for 2 successive weeks.The rats＇ body weight,sucrose consumption volume in the sucrose preference test,and times of grooming in the open field test were detected on the 0,7th,14th,21st,28th day,respectively.The mRNA expressions of BDNF and TrkB in the hippocampus,amygdala,and the frontal lobe were detected by immunohistochemical assay and Real-time fluorescent quantitation PCR.Results Compared with the NC group,the rats＇ body weight was put up slowly in the MC group.The scores in the open field test decreased.The times of grooming and sucrose consumption volume were both reduced.The time of staying in central square was postponed.The mRNA expressions of BDNF and TrkB in the hippocampus,amygdala,and the frontal lobe decreased with statistical significance（P0.05,P0.01）.Compared with the MC group,the behavior indices of rats in the CHSGP,CⅠ,CⅡ,and FC groups were significantly improved.The mRNA expressions of BDNF and TrkB in the hippocampus,amygdala,and the frontal lobe were obviously enhanced with statistical significance（P0.05,P0.01）.Conclusions CHSGP could obviously improve the depressive state of the model rats.Its mechanism might be correlated with increasing the mRNA expressions of BDNF and TrkB in the hippocampus,amygdala,and the frontal lobe.