胎儿膀胱发育中肥大细胞量变的探讨

目的 :探讨肥大细胞在人胎儿膀胱发育中的数量变化。材料和方法 :43例不同胎龄的人胎儿膀胱切片做甲苯胺蓝 (TB)和阿尔辛蓝 藏红 (AB S)染色并对肥大细胞进行计数和用方差分析做统计学处理。结果 :胎儿膀胱肥大细胞 3月龄时开始出现 ,数量为 3 7.1 8± 5 .72个 /mm2 ,6月龄时增长迅速 ,为 1 61 .48± 4.3 8个 /mm2 ,约是最初量的 5倍 ,该变化具有统计学意义 (P <0 .0 1 )。 9月龄时肥大细胞数量可达 2 3 7.92± 5 .48% ,约为最初量的 7～ 9倍。结论 :在人胎儿膀胱肥大细胞在 6月龄前后明显增多 ,并且随着胎龄增长而进一步增多。

QUANTITATIVE STUDY OF VARIATION OF MAST CELL NUMBER IN HUMAN FETUS DURING BLADDER DEVELOPMENT

Objective: To study the variation of the mast cell number in human fetus in the course of the bladder deveolpment. Methods: The sectionsof bladder from 43 human fetuses at different gestation age were stained with Toluidine blue(TB) and Alcian blue-safranin(AB-S) mixutre respectively., then mast cell number was counted and analyzed statistically by analysis of variance. Results: In the fatuses at 3 months, a few mast cells began to be found and their number was 37.18 ±+ 5.72/mm2, whereasduring the 6th month, the number of mast cells increased greatly and was 161.48 + 4.38/mm2, beingabout 5 times of the primitive quantity, the varation was significant(P ＜ 0.01 ). Approach to delivery,the amount of mast cells reached 237.92 + 5.48/mm2, which was 7 to 9 times of the primitive.Conclusion: During bladder development of human fetus, the number of mast cells increases greatly as the fetus grows, especially before or after 6 months.