1，2-二氯乙烷生物标志的研究

目的]探讨1，2-二氯乙烷在尿中的代谢产物作为生物标志物，为预防1，2-二氯乙烷职业中毒提供依据。方法]将SD大鼠分为染毒组和对照组，每组2只。用1，2-二氯乙烷对大鼠作灌胃染毒，然后收集尿样，经酸化、蒸发及甲酯化处理后用气相色谱-质谱（GC-MS）分析。结果]在染毒大鼠的尿样中检出6种化合物：硫撑双乙酸二甲酯、2-甲硫基乙酸甲酯、磷酸三甲酯、2-甲氧基．2-戊烯二酸二甲酯、柠檬酸三甲酯和3，4-二甲氧基苯丙酸甲酯。其中硫撑双乙酸二甲酯和柠檬酸三甲酯的色谱信号较高，硫撵双乙酸已有文献报道是1，2．二氯乙烷代谢产物。结论]硫撑双乙酸二甲酯作为接触1，2-二氯乙烷的生物标志物，值得进一步研究。柠檬酸三甲酯异常增高，可能与阻断三羧酸循环有关，有可能可作为1，2-二氯乙烷中毒的初步排查标志。

Study on Biomarker of 1, 2-dichloroethane

Objective] The urinary metabolites of 1,2-dichloroethane were studied and biomarkers of exposure to 1,2-dichloroethane were discussed to provide evidence for preventing occupational poisoning of 1,2-dichloroethane.Methods] Four SD rats were divided into exposed group and control group,with two rats in each group.After 1,2-dichloroethane administered to rats via gavage,urine was collected and the urinary metabolites were analyzed by Gas Chromatography-Mass Spectrometry(GC-MS)with pretreatment of acidificat...