人脐带血细胞分化为神经样细胞的实验研究

为探讨人脐带血细胞分化为神经样细胞的可行性 ,无菌条件下采集正常人脐血 5 0～ 10 0mL置于加有抗凝剂的采血袋中 ,分离脐血单个核细胞进行培养 ,并加入细胞因子加以诱导分化 ,利用免疫细胞化学方法进行鉴定。结果显示 :脐血单个核细胞能在体外培养中增生分化和表达干细胞特异性抗原神经巢蛋白 (nestin) ,并最终分化为神经元样和神经胶质样细胞。免疫组化染色可见神经元特异性烯醇化酶 (NSE)和胶质原性纤维酸性蛋白 (GFAP)抗原表达。碱性成纤维细胞生长因子 (bFGF)和表皮生长因子 (EGF)协同作用对脐血细胞的分化有促进作用。提示 :脐血单个核细胞具有增生和分化潜能 ,可分化为神经元样及神经胶质细胞样细胞 ,可以作为神经细胞的种子细胞。

Expansion and Differentiation of Human Umbilical Cord Blood Cells Into Neuronlike Cells

The aim was to explore the isolation, purification and differentiation of umbil ical cord blood cells into neuronlick cells in vitro. Human cord blood samples were obtained, cultured and induced with fectors. Identification was performed with immunochemistry method. Umbilical cord blood could proliferate an d generate clones when cultured in vitro. These cell grew rapidly and different iated into neuronlike cells and astrocytelike cells. The cell clones proliferate d from stem cell could express nestin antigen and the differentiated cells expre ssing GFAP or NSE antigen respectively. In this study, bFGF and EGF in fluencing the induction and differentiation of Umbilical cord blood were observe d. It suggests that umbilical cord blood cells have selfrenewal and differentiat ion abilities. They might differentiate into neuronlike and astrocytelike cells which express GFAP or NSE. The umbilical cord blood can be considered the ideal seed cells of the neural stem cells.