薯蓣皂苷元对人胃癌BGC-823和SGC-7901细胞生物学行为的影响

目的: 观察薯蓣皂苷元对人胃癌BGC-823和SGC-7901细胞增殖、侵袭、迁移和凋亡过程的影响并探讨其机制。方法: 采用薯蓣皂苷元处理体外培养的BGC-823和SGC-7901细胞，用MTT法、Transwell 实验检测细胞的增殖、迁移、侵袭能力。用免疫印迹法检测BGC-823和SGC-7901细胞中凋亡相关蛋白BAX、凋亡抑制基因Bcl-2和MAPK信号通路的Erk1/2、JNK、p38三个通路中相关蛋白的表达。结果： 经薯蓣皂苷元处理后，BGC-823和SGC-7901细胞的增殖、迁移和侵袭能力明显降低，凋亡相关蛋白BAX明显升高，Bcl-2的表达明显降低；p-p38表达水平明显降低，但JNK、p-JNK、Erk1/2、p-Erk1/2和p38的表达无明显变化。结论： 薯蓣皂苷元可能通过MAPK通路中的p-p38通路影响人胃癌BGC-823和SGC-7901细胞的生物学行为。

Diosgenin affect human gastric cancer BGC-823 and SGC-7901 cells through MAPK pathways

Objective: To discuss the role of diosgenin in the proliferation, invasion，migration and apoptosis of human gastric cancer BGC-823 and SGC-7901 cells via MAPK signaling pathways. Methods: Human gastric cancer cell lines BGC-823 and SGC-7901 were cultured in vitro and treated with diosgenin. Proliferation rate, cell migration and invasion were measured by MTT method via Transwell assay. And protein expression of apoptosis-associated protein (AAP) BAX, apoptosis inhibitor protein Bcl-2 and Erk1/2,JNK and p38 proteins of MAPK pathways were measured by Western Blot. Results: When BGC-823 and SGC-7901 cells were treated with diosgenin，the proliferation, migration and invasion of BGC-823 and SGC-7901 cells were significantly decreased. The apoptosis of both cells was enhanced to a certain extent. As to the correlation of gastric cancer cells and MAPK pathways，we found that p-p38 protein expression level after diosgenin treated was dramatically down-regulated; however，the expression levels of Erk1/2，JNK，p38，p-Erk1/2 and p-JNK were negligible. Conclusion: Diosgenin might affect the proliferation，invasion，migration and apoptosis of human gastric cancer BGC-823 and SGC-7901 cells through p-p38 of MAPK pathways.