来源于成纤维细胞激活蛋白α的多肽疫苗FAPτ的抗肿瘤效应

目的 了解来源于成纤维细胞激活蛋白α的多肽疫苗 – FAPτ的抗肿瘤效应。方法 将抗原FAPτ与氟氏不完全佐剂混合均匀，在第1、14、21天于小鼠背部皮下多点注射进行免疫，于第17天给小鼠接种肿瘤细胞，记录小鼠肿瘤体积变化曲线及存活率曲线。肿瘤接种22天后对小鼠进行尾静脉取血并取脾脏分离脾脏淋巴细胞。ELISA检测小鼠血清内FAPτ抗体滴度；脾脏淋巴细胞体外经抗原FAPτ再次刺激，流式细胞仪检测CD8⁺ T细胞内IFN-?γ的产生。结果 FAPτ免疫组小鼠肿瘤生长速度低于PBS对照组，存活率高于PBS对照组。FAPτ 免疫组小鼠血清内产生较高的抗体滴度；其脾脏CD8⁺ T细胞内有更高水平的IFN-?γ表达。结论 多肽疫苗FAPτ能够有效激活机体的体液免疫应答和CD8⁺ T细胞免疫应答，显示出较强的抗肿瘤活性。

Anti-tumor Effect of Polypeptide Vaccine FAPτ from Fibroblast Activation Protein α

Objective To detect the anti-tumor effect of polypeptide vaccine-FAPτ ?from fibroblast activationprotein α. Methods The antigen FAPτ?was mixed uniformly with Freund’s incomplete adjuvant and thenimmunized in multiple locations on the back of mice on day 1, 14 and 21. Tumor cells were implanted into themice on day 7. Curves of tumor volume change and mice survival were recorded. Murine serum and splenic cellswere collected on day 22 after the implantation. Antibody titer of FAPτ ?was detected by ELISA. IFN-?γ expressionin CD8⁺T cells was detected by flow cytometer. Results The tumor growth rate was reduced while thesurvival rate was increased in mice immunized with FAPτ ?vaccine compared that in with PBS control group.Similarly, high level of antibody titer and IFN-γ expression in CD8⁺T cells were detected in mice immunizedwith FAPτ.Conclusion By activating humoral immune response and CD8⁺T cell immune responseeffectively, FAPτ?has strong anti-tumor activity.