集落刺激因子-1及其受体对乳腺癌荷瘤鼠肿瘤生长的影响

目的 探讨CSF-1及其受体对乳腺癌荷瘤鼠肿瘤体积以及对其免疫器官包括骨髓、胸腺、淋巴结和脾的影响。 方法 将4T1细胞（1×107/只）重悬于PBS液接种于Balb/c 小鼠左腋皮下，建立荷乳腺癌小鼠模型为实验组，同时培养相同条件的对照组。检测成瘤率，测量肿瘤体积的变化并绘制成图；成瘤后为实验组小鼠注射CSF-1及其受体，观察一段时间后同时处死两组小鼠，采用半定量RT-PCR技术检测鼠脾脏中CSF-1的表达；采用MTT比色法检测小鼠的肿瘤、脾脏、骨髓中细胞的增殖功能。 结果 4T1细胞接种后小鼠的成瘤率为100%，成瘤潜伏期平均为7~10天；实验组小鼠瘤体的增长速度快于对照组；RT-PCR的电泳结果显示，实验组小鼠的脾脏CSF-1的mRNA的表达量上升；MTT结果表明，CSF-1作用之后的实验组小鼠的肿瘤细胞和免疫细胞增殖活性升高，发挥了强大的免疫抑制功能，促进肿瘤的增长；增殖活跃的骨髓和脾细胞呈现免疫抑制表型。 结论 CSF-1及其受体对乳腺癌荷瘤鼠的肿瘤的生长有促进作用。

Infl uence of Colony Stimulating Factor-1 and Its Receptor on Cancer Cells and Immune Cells of Breast Cancer Mice

Abstract: Objective To study the influence of colony stimulating factor-1(CSF-1) and its receptor oncancer cells and immune cells of breast cancer mice and to explore their effects on tumor size, volume andimmune system including marrow, thymus, lymphonodus and spleen. Methods 4T1 cell (1×107/PBS) wasinoculated in the left subcutaneous of Balb/c mice in order to establish the mice model with breast cancer.Then tumor formation rate, average weight and area change of tumor were measured. CSF-1 and its receptorwere injected into experimental mice after tumor was formed, and these mice were killed after observation.The expression of CSF-1 in the spleen of mice was tested by semi-quantitative RT-PCR. The proliferationof T lymphocyte in the spleen and bone marrow were detected by MTT colorimetry. Results After thecontinuous improvement, tumor formation rate of 4T1 breast cancer was 100% and tumor latency was 7-10days. After the injection of CSF-1, we found that tumor growth rate of the experimental mice was higher thanthat of control group. RT-PCR and electrophoresis results revealed that expression of CSF-1 was increasedin the spleen of tumor-bearing mice.MTT results showed that the proliferation of immune lymphocytesand tumor cells was increased after CSF-1 treatment. Active proliferation of bone marrow and spleen cellsexhibited immunosuppressive phenotype. Conclusion CSF-1 and its receptor could promote the growth ofbreast cancer tumor in mice.