穴位注射胞二磷胆碱对脑外伤大鼠生长相关蛋白-43 表达的影响

目的观察足三里穴位注射胞二磷胆碱疗法对脑外伤大鼠神经功能及生长相关蛋白(GAP-43)表达的影响。方法成年健康雄性Sprague-Dawley大鼠40 只，除假手术组大鼠8 只(A组)外，其余大鼠采用改良Feeney 法建立大鼠脑损伤模型，造模成功大鼠分为穴位注药组(B 组)、穴位注水组(C 组)、腹腔给药组(D组)和腹腔注水组(E 组)各8 只，造模后每日分别给予足三里穴位注射胞二磷胆碱或生理盐水，腹腔注射胞二磷胆碱或生理盐水，连续14 d。于伤前及伤后第8、14、15、22 天分别行神经功能缺损评分及旷场实验，28 d 后采用免疫组化方法检测大鼠脑组织GAP-43 水平。结果B组大鼠神经缺损评分改善和旷场试验评分均优于C、D、E组(P<0.05)。B组大鼠脑组织GAP-43 阳性表达多于C、D、E组(P<0.05)。结论足三里穴位注射胞二磷胆碱可促进脑外伤大鼠GAP-43 蛋白表达，促进神经功能改善。

Effects of Citicoline Acupoint Injection on Expression of Growth Associated Protein-43 in Rats with Traumatic Brain Injury

Objective To investigate the effects of injection of citicoline into Zusanli (ST36) acupoint on neural function and expression of growth associated protein-43 (GAP-43) in rats with traumatic brain injury. Methods 40 healthy adult male Sprague-Dawley rats were randomly divided into 5 groups: sham-operated group (group A, n=8), acupoint injection of citicoline group (group B, n=8), acupoint injection of saline group (group C, n=8), intraperitoneal injection of citicoline group (group D, n=8) and intraperitoneal injection of saline group (group E, n=8). Opened brain trauma was induced with the modified Feeney method in the groups B, C, D and E, and were treated as design, once a day for 14 days. They were assessed with nervous function score and open-field test before and 8, 14, 15, and 22 days after injury. The expression of GAP-43 in the brain were detected with immunohistochemistry 28 days after injury. Results The nervous function scores and open-field test scores improved more significantly in the group B than in the groups C, D and E (P<0.05). The expression of GAP-43 increased in the group B than in the groups C, D and E (P<0.05). Conclusion Acupoint injection of citicoline into Zusanli may improve the expressions of GAP-43 to promote the recovery of neural function in rats after traumatic brain injury.