鳄嘴花正丁醇提取物对小鼠Heps肝癌的抑制作用

目的: 研究鳄嘴花正丁醇提取物Clinacanthus nutans (Burm.f.) Lindau n-butanol extracts，CN-N\]对荷瘤小鼠的体内抑瘤作用及延长生存时间作用。方法: 肝癌细胞接种至ICR小鼠右侧腋下，建立Heps小鼠肝癌模型，随机分成4组，对照组、环磷酰胺组、CN-N低剂量组(3 mg·kg-1·d-1)、CN-N高剂量组(10 mg·kg-1·d-1)，每组10只，观察CN-N对荷瘤小鼠的抑瘤率、体质量、脾脏及胸腺指数、生存时间的影响。蛋白质印迹法检测增殖细胞核抗原(proliferating cell nuclear antigen，PCNA)的表达，TUNEL法检测细胞凋亡情况。结果： 与对照组比较，CN-N低剂量组和高剂量组肿瘤质量明显降低(t分别为2.261，3.140，P均＜0.05)，荷瘤小鼠脾脏指数、胸腺指数差异无统计学意义(P均＞0.05)，PCNA表达均明显减少；CN-N高剂量组生命延长率为54.8%，较对照组明显延长荷瘤小鼠生存期(t=3.416，P=0.003 1)。结论： 鳄嘴花正丁醇提取物具有良好的抗肿瘤作用，高剂量CN-N能提高荷瘤小鼠的生存期。

Inhibitive effect of Clinacanthus nutans (Burm.f.) Lindaun-butanol extracts on Heps hepatoma in mice

Objective: To investigate the anti tumor activity and life extention effect of Clinacanthus nutans (Burm.f.) Lindau n-butanol extract (CN-N) on tumor loaded mice. Methods:Mice hepatoma carcinoma cells (Heps, grown in donor mice) were transplanted subcutaneously into armpit of the ICR mice to establish the tumor xenografts model. Tumor mice were randomized into four groups: control group, cytoxan group, CN-N low dose group (3 mg·kg-1·d-1), CN-N high dose group (10 mg·kg-1·d-1). Tumor inhibition rate, body weights, immune organs index and life prolonging effect were recorded. Proliferation of tumor cells were detected by western blotting and apoptosis was examined by TUNEL assay. Results: Compared with the control group, CN-N low dose group and high dose group showed remarkable inhibition of tumor weight (t=2.261, 3.140, both P＜0.05);no significant differences on spleen and thymus index of tumor earing mice(both P＞0.05). PCNA expression levels were greatly reduced when treated with low or high concentration of CN-N. Compared with the control group, CN-N high dose group significantly extended the survival time(t=3.416, P=0.003 1). Conclusion: CN-N exhibited good antitumor activity and significantly prolonged the survival time of the tumor-bearing mice.