秦皮乙素对BALB/c裸鼠体内胶质瘤生长的影响

摘 要: 【目的】 探讨秦皮乙素(Esc)的体内抗胶质瘤活性,为秦皮乙素进一步研究开发为抗胶质瘤药物提供实验依据?【方法】 采用C6细胞前肢腋下皮下注射BALB/c裸鼠种瘤构建荷瘤鼠模型;分为4组:模型组?50 mg/kg秦皮乙素组?100 mg/kg秦皮乙素组和阳性对照组;采用瘤体积?相对肿瘤增殖率?瘤质量?肿瘤生长抑制率和体质量5个指标评价秦皮乙素的体内抑瘤效果;采用HE染色鉴定瘤体?【结果】 与模型组相比,秦皮乙素可显著抑制雄性荷瘤鼠的瘤体积约10倍,瘤质量约8倍,对其体质量无显著影响,与阳性对照药尼莫司汀(ACNU)相似;对雌性荷瘤鼠瘤体的生长无显著抑制作用?【结论】 秦皮乙素在雄性荷瘤鼠体内具有显著抗胶质瘤活性,为秦皮乙素进一步开发为抗胶质瘤药物提供了实验依据?

The Effect of Esculetin on Glioma Growth in BALB/c Nude:An in Vivo Study

Abstract:【Objective】 This study aimed to investigate the in vivo anti-glioma activity of Esculetin (Esc) to provide experimental evidences for further study of Esculetin as a potential anti-glioma drug. 【Method】 Rat glioma C6 cell was subcutaneously injected into BALB/c nude mouse to set up the tumor-bearing mouse model. Four groups were taken in this experiment: model group, 50 mg/kg Esculetin group, 100 mg/kg Esculetin group and positive control group. Tumor volume, relative tumor proliferation rate, tumor weight, inhibition rate and body weight were measured to evaluate the in vivo anti-glioma activity of Esculetin. HE staining was used to identify the tumor tissue. 【Results】 Comparing with the model group, Esculetin significantly inhibited tumor volume to 1/10, and tumor weight to 1/8 of the model group in male tumor-bearing mouse, similar to the positive control drug Nimusting (ACNU), while showed no significant anti-glioma activity in female tumor-bearing mouse.【Conclusion】 Esculetin exhibits significant anti-glioma activity on male tumor-bearing mouse, thus provides experimental evidences for further study of Esculetin as a potential anti-glioma drug.