过表达外源FUT7对乳腺癌细胞黏附及迁移能力的影响

目的 观察过表达外源岩藻糖基转移酶VII（FUT7）对人乳腺癌MDA-MB-231细胞黏附和迁移能力的影响。方法 构建以FUT7为靶基因的真核表达载体pEGFP-C1-FUT7，转染人乳腺癌MDAMB-231细胞，采用RT-PCR检测FUT7 mRNA的表达；Western blot检测FUT7及其催化产物sLeX蛋白水平；细胞黏附实验及Transwell小室检测转染后细胞与HUVECs细胞黏附、迁移能力。结果 转染细胞外源FUT7表达在mRNA水平和蛋白水平及sLeX含量均明显增加；转染的乳腺癌细胞黏附和迁移能力明显增强。 结论 过表达外源FUT7基因可增强人乳腺癌MDA-MB-231细胞黏附和迁移能力。

Effects of Overexpression of Exogenous FUT7 on Adhesion and Migration of Breast#br# Cancer Cell

Objective To investigate the effect of exogenous overexpression of α1,3 FucT-Ⅶ(FUT7) onadhesion and migration of human breast cancer cell line MDA-MB-231. Methodes pEGFP-C1-FUT7eukaryotic expression vector was constructed and transfected them into MDA-MB-231 cells. The expressionlevel of FUT7 mRNA was examined by RT-PCR; Expression of FUT7 and catalysate sLeX protein wereassayed by Western blot. Infl uence of exogenous FUT7 overexpression on adhesion and migration of MDAMB-231 cells to HUVECs was analyzed by adhesion assay and transwell assay. Results The exogenousexpression levels of FUT7 mRNA，sLeX protein on cell surface were signifi cantly increased in transfectedMDA-MB-231 which had enhanced abilities of adhesion and cell migration. Conclusion Overexpressionof exogenous FUT7 promoted the abilities of adhesion and migration of human breast cancer cell line MDAMB-231.