洛铂对骨肉瘤MG-63细胞增殖、凋亡的影响及其机制

目的 探讨洛铂对骨肉瘤MG-63细胞增殖与凋亡的作用及其机制。方法 取对数生长期的MG-63细胞进行实验，分为对照组和实验组 (加入不同浓度洛铂: 2、4和8 μg/ml)。采用噻唑盐（MTT）比色法、流式细胞技术(FCM)检测洛铂对MG-63细胞的形态改变、生长及增殖抑制、细胞周期阻滞和细胞凋亡诱导等作用，并绘制不同浓度时MG-63细胞的生长曲线，应用Western blot的方法分析洛铂对MG-63细胞的Bcl-2蛋白表达的影响。结果 洛铂能够抑制MG-63细胞的生长、增殖并诱导其凋亡，并呈浓度和时间依赖效应。Western blot检测结果显示洛铂可使MG-63细胞Bcl-2蛋白表达下降。结论 洛铂能显著抑制MG-63细胞增殖，诱导细胞凋亡和细胞周期变化，可能与调节 MG-63细胞Bcl-2蛋白的表达有关。

Effects of Lobaplatin on Proliferation and Apoptosis of Osteosarcoma MG-63 Cells and Its Mechanism

Objective To study the effects of lobaplatin on the proliferation and apoptosis of osteosarcomaMG-63 cells and the mechanism of its function. Methods MG-63 cells in log phase were divided intocontrol group and experimental groups. Experimental groups were treated with different concentrations oflobaplatin (2, 4 and 8μg/ml). MTT colorimetry and fl owcytometry were used to detect the effects of lobaplatinon cellular morphology change, cell growth, cell cycle, and the induction of apoptosis in MG-63 cells. Cellgrowth curves were studied at different concentrations of lobaplatin. Western blot was used to detect theeffects of lobaplatin on the expression of Bcl-2 in MG-63 cells. Results Lobaplatin could inhibit the growthor proliferation and increase the apoptosis of osteosarcoma cell line MG-63 in a dose- and time-dependentmanner. Bcl-2 protein level was decreased after lobaplatin treatment. Conclusion The effects of lobaplatinon the proliferation, the apoptosis and changing the cell cycle change of MG-63 cells might be related to itsinhibition on Bcl-2 protein expression.