类风湿关节炎患者外周血单个核细胞中糖酵解相关基因的表达

目的: 探讨糖酵解相关基因在类风湿关节炎(rheumatoid arthritis,RA)患者外周血单个核细胞（peripheral blood mononuclear cells，PBMC）中的表达及其意义。方法: 选取活动期RA患者19例，稳定期RA患者23例，采用Ficoll法分离PBMC，定量PCR检测糖酵解相关基因表达水平，并与健康对照进行比较。结果：与健康对照相比，活动期RA患者PBMC中糖酵解相关基因丙糖磷酸异构酶（triosephosphate isomerase，TPI）、烯醇化酶（enolase，ENO）、M型丙酮酸激酶（pyruvate kinase muscle，PKM）、单羧酸转运蛋白（monocarboxylic acid transporter member，MCT）表达量均增加(P<0.05)，稳定期RA患者4种基因的表达略有升高，但差异无统计学意义。结论：RA患者存在糖酵解相关基因表达的改变。糖酵解反应有可能在RA疾病机制中具有重要的意义。

Expression of glycolysis related gene in peripheral blood mononuclear cells of patients with rheumatoid arthritis

Objective: To explore the mRNA expression and potential significance of glycolysis related gene in peripheral blood mononuclear cells(PBMC) of patients with rheumatoid arthritis(RA). Methods: PBMC were purified by Ficoll and glycolysis related genes mRNA expression was analysed by real-time PCR. Results: Compared with healthy control, PBMC from active RA patients significantly expressed higher level of glycolysis related genes including TPI，ENO，PKM and MCT. However，there were no significant differences in these genes between inactive group and control group. Conclusion: In RA patients, glycolysis related genes mRNA expression level were changed. Glycolysis may play an important role in the mechanism of RA.