淋巴结转移乳腺癌患者中RARβ基因甲基化的预后判断价值

RARβ Gene Methylation Could Be Used in Prognosis of Breast Cancer Patients with Lymphatic Metastasis

Objective To determine the roles of retionoic acid receptor β(RARβ) gene methylation in theoccurrence and development of breast cancer. Methods We screened the primary human breast tumors andnormal breast tissues for RARβ gene promoter methylation by methylation specify PCR(MSP), and the resultswere analyzed together with corresponding clinical pathological data. Results The frequency of RARβ genemethylation in 192 cases was 26% (50/192), however no RARβ gene methylation was found in normal breasttissues. RARβ methylation were associated with tumor histological type, staging and tumor size (P<0.05).Patients with lymph node metastasis, ER（-）and HER2 amplified had more RARβmethylation(P>0.05).Patients with RARβ methylation had low survival rate(P=0.268), particularly those combined with lymphaticmetastasis (P=0.04). Conclusion RARβ gene promoter methylation may play an important role in thecarcinogenesis and development of breast cancer. The relationship with poor differentiation, large tumorsize and poor survival rate indicates that RARβ methylation could be used in the prognosis of breast cancerpatients with lymphatic metastasis.