肌筋膜经线选穴法针刺治疗对脑性瘫痪模型大鼠神经细胞再生及运动功能恢复的影响

目的探讨基于肌筋膜经线的选穴法针刺治疗对脑瘫模型大鼠神经细胞再生及运动功能恢复的影响。方法健康21日龄雄性Sprague-Dawley 大鼠20 只，分成对照组、针刺1 组、针刺2 组和假手术组，对照组、针刺1 组和针刺2 组采用左侧颈总动脉结扎结合低氧环境制作脑瘫模型。针刺1 组接受传统针刺疗法，针刺2 组接受基于肌筋膜经线的选穴法针刺治疗。各组于针刺开始后第3、7、14 天行BBB评分、斜板试验；第14 天用5-溴脱氧尿核苷(BrdU)标记检测左脑运动皮层和纹状体的细胞增殖情况。结果第7 天和第14 天针刺2 组的BBB评分恢复明显优于针刺1 组和对照组(P<0.01)，第3、7、14 天针刺2 组斜板支撑能力均较对照组升高(P≤0.05)；第14 天针刺2 组运动皮层BrdU含量较针刺1 组升高(P<0.05)。结论基于肌筋膜经线的选穴法针刺治疗能有效促进脑瘫模型神经细胞再生和运动功能的改善，且优于传统针刺疗法。

Effects of Acupuncture Based on Acupoint Selection of Myofascial Meridians Perspective on Regeneration of Nerve Cells and Recovery of Motor Function in Rats with Cerebral Palsy

Objective To investigate the effects of acupuncture based on acupoint selection of myofascial meridians perspective on the regeneration of nerve cells and recovery of motor function in rats with cerebral palsy. Methods 20 healthy 21-day-old male Sprague-Dawley rats were divided into control group, acupuncture groups 1 (receiving traditional acupuncture) and 2 (receiving acupuncture based on acupoint selection of myofascial meridians perspective), and sham group. The control group and the acupuncture groups were subjected to ligation of left carotid artery (ischemia) and then put into a hypoxic environment. They were assessed with Basso-Beattie-Bresnahan (BBB) Scale and inclined plane test 3 d, 7 d and 14 d after the beginning of acupuncture. And 5-bromo-2'-deoxyuridine (BrdU) was used to label Sphase cells in the left striaturn and motor cortex in each group 14 d after the beginning of acupuncture. Results The BBB scores improved more in the acupuncture group 2 than in the acupuncture group 1 and the control group 7 d, 14 d after the beginning of acupuncture (P<0.01), as well as the score of inclined plane test 3 d, 7 d, 14 d after acupuncture (P<0.05). BrdU was more in the left motor cortex in the acupuncture group 2 than in the acupuncture group 1 (P<0.05). Conclusion Acupuncture based on acupoint selection of myofascial meridians perspective can promote the regeneration of nerve cells and recovery of motor function in rats with cerebral palsy, that is more effective than traditional acupuncture.