Nickel-induced plasma lipid peroxidation and effect of antioxidants in human blood: involvement hydroxyl radical formation and depletion of alpha-tocopherol |
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Authors: | Chen Chang-Yu Su Yuh-Juan Wu Pey-Fen Shyu Mei-Miin |
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Affiliation: | Department of Medical Technology, Foo Yin Institute of Technology, Kaoshiung, Taiwan, Republic of China. ccy1689@ms32.hinet.net |
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Abstract: | To provide evidence for the oxidative effect of nickel (Ni) treatment on blood, lipid peroxidation (LPO) and hydroxyl radical (*OH) generation were examined in human plasma. Nickel chloride induced LPO in plasma of human blood in vitro in a concentration-dependent (0-10 mM) and time-dependent (0-2 h) manner. The *OH production in plasma was quantified by measurement of conversion of salicylic acid (SA) into its hydroxylated products, 2,3- and 2,5-dihydroxybenzoate (DHB). The concentrations of 2,3- and 2,5-DHB in plasma increased in a concentration-dependent manner after Ni treatment for 1 h. Furthermore, a decreasing trend in alpha-tocopherol levels in plasma was observed after Ni treatment. Concurrent incubation with gluthathione (GSH), catechin (CTCH), and mannitol decreased lipid peroxidation and reduced *OH formation induced by Ni, but exacerbation of the decrease of alpha-tocopherol in plasma occurred with catechin. |
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