| Neurocyte Apoptosis and Expressions of Caspase-3 and Fas after Spinal Cord Injury and Their Implication in Rats |
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| 作者姓名： | 王金光 郑启新 赵铭 郭晓东 |
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| 作者单位： | Department of Orthopedics Union Hospital Tongji Medical College of Huazhong University of Science and Technology，Department of Orthopedics Union Hospital Tongji Medical College of Huazhong University of Science and Technology，Department of Orthopedics Union Hospital Tongji Medical College of Huazhong University of Science and Technology，Department of Orthopedics Union Hospital Tongji Medical College of Huazhong University of Science and Technology，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China |
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| 摘 要： | Spinal cord injury (SCI) is a serious injury com-monly seen in surgical patients, for which there is no established treatment available at present. After spinal injury, neurocytes and tissues undergo necrosis and apoptosis. Apoptosis is a complex pathophysilogical process, mainly involving protease cascade mediated by the members of caspase family, among which caspase-3 plays a key role. Caspase-3 (CPP32) belongs to the cystine-aspartic proteinase family and plays the central role in the exe…
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| 关 键 词： | 神经细胞 细胞凋亡 神经损伤 基因 |
| 收稿时间： | 2006-05-23 |
| 修稿时间： | 2006-05-23 |
Neurocyte apoptosis and expressions of capase-3 and Fas after spinal cord injury and their implication in rats |
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| Jinguang Wang,Qixin Zheng,Ming Zhao,Xiaodong Guo.Neurocyte Apoptosis and Expressions of Caspase-3 and Fas after Spinal Cord Injury and Their Implication in Rats[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(6):709-712. |
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| Authors: | Jinguang Wang Qixin Zheng Ming Zhao Xiaodong Guo |
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| Institution: | Department of Orthopedics, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430022, China |
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| Abstract: | To study the expression of neurocyte apoptosis and the changes of caspase-3 and Fas after spinal cord injury (SCI) in rats, improved Allen's method was used to make model of acute SCI at the level of T9 and T10. The animals were divided into six groups: a control group and 5 injury groups. The segments of injured spinal cords were taken 6, 24, 48 h and 7, 15 days after injury for morpho-logical studies, including HE staining, Hoechst33258 staining and TUNEL labeling. The expression of caspase-3 was detected by immunohistochemical staining and RT-PCR. TUNEL-positive cells be-gan to appear in the compression region 6 h after the injury, mostly located in the gray matter. TUNEL-positive cells were found in both gray and white matter, reaching a peak at the 3rd day. They began to decrease at the 7th day, distributed mostly in the white matter. Fas increased at the 6th h and peaked at the 3th day. Caspase-3 mRNA increased at the 6th h, peaking 48 h after the trauma, and de-creased after 7 days. The protein expression of caspase-3, as revealed by immunohistochemical staining, was similar to TUNEL in time. It is concluded that apoptosis takes place after spinal cord injury, and caspase-3 mRNA and protein expressions were enhanced in the apoptosis. The expression of caspase-3 has a positive correlation with Fas expression. |
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| Keywords: | spinal cord injury apoptosis caspase-3 Fas |
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