PMEPA1在前列腺癌中的表达及临床意义

 目的 探讨一种编码受雄激素诱导的前列腺跨膜蛋白基因——PMEPA1（transmembrane prostate androgen induced RNA）的表达，预测前列腺癌非激素依赖型变化的临床意义。 方法 采用实时荧光定量PCR检测PMEPA1及GSTP1 mRNA在前列腺癌细胞系LNCaP、PC-3、良性前列腺增生上皮细胞及33例前列腺癌和16例前列腺增生组织中的表达情况。 结果 GSTP1及PMEPA1在前列腺癌细胞系中的表达均下调。GSTP1在6.1%的前列腺癌患者中表达上调，在81.8%的患者的前列腺癌组织中表达下调，在12.1%的患者中表达无明显差异；PMEPA1在27.3%的前列腺癌患者的前列腺癌组织中表达上调，在27.3%的患者中表达下调，在45.4%的患者中表达无明显差异。统计分析表明，GSTP1的表达与年龄有关，与PSA、TNM分期、有无骨转移及分化程度无显著关系；PMEPA1的表达与肿瘤的分化程度及是否有骨转移有关，而与年龄、PSA、TNM分期无显著关系。结论 PMEPA1有可能作为区分临床预后较差的前列腺癌的分子学标记，但需要进一步做大样本的研究。

The clinical significance of the expression level of PMEPA1 in prostate cancer

Objective To study the clinical significance of the mRNA expression level of a novel gene which encodes a kind of transmembrane prostate protein induced by androgen-PMEPA1, as it may predict the progress of prostate cancer from hormone-dependent to hormone-independent. Methods We used Real-time PCR to detect the mRNA expression of PMEPA1 and GSTP1 in prostate cancer cell lines (LNCaP, PC-3), epithelia cells of benign prostatic hyperplasia and tissues from 33 patients with prostate cancers and 16 cases of prostatic hyperplasia. Results We found the mRNA expression of GSTP1 and PMEPA1 were both down-regulated in prostate cancer cell lines. The mRNA expression of GSTP1 was up-regulated in 6.1% of cases, down-regulated in 81.8%, and showed no difference in 12.1%. While PMEPA1 was highly expressed in 27.3% of cases, lowly expressed in 27.3%, and not differently expressed in 45.4%. Statistical analysis showed that the mRNA expression of GSTP1 was relevant to ages, but had no relationship with PSA, TNM stage, osseous metastasis or tumor differentiation, while the mRNA expression of PMEPA1 was relevant to osseous metastasis and tumor differentiation, but had no relationship with age, PSA or TNM stage. Conclusions PMEPA1 is possibly a useful biomarker, as it can identify patients with unfavourable prognosis, however, this hypothesis needs to be further studied with large samples.