酸枣仁与缅枣仁的蛋白质分析

目的：初步探讨酸枣仁与缅枣仁的蛋白质差异。方法：用凯氏定氮法测定蛋白质的含量；用酸水解法测定蛋白质的氨基酸组成；用十二烷基硫酸钠－聚丙烯酰胺凝胶电泳（SDS－PAGE）和毛细管区带电泳（CZE）分析蛋白质的组分。结果：酸枣仁与缅枣仁的蛋白质含量分别为36．13％和41．58％；除缅枣仁蛋白质的缬氨酸和蛋氨酸明显高于酸枣仁外，蛋白质的其他氨基酸的组成差别不明显；SDS－PAGE图谱明显不同的是，酸枣仁有相对分子质量为39800的蛋白质，缅枣仁为50100的蛋白质；CZE图谱的区别是，酸枣仁为3．1min的峰，缅枣仁含有一个迁移时间为4．8min的峰。结论：缅枣仁的蛋白质含量较酸枣仁略高，且缬氨酸和蛋氨酸的含量明显高于酸枣仁。两种枣仁的SDS－PAGE和CAE图谱均有明显的差异存在。

Analysis of proteins in seed of spine date and seed of Indian jujube

Object To preliminarily study the difference of proteins in the seed of spine date and the seed of Indian jujube. Methods Protein contents were measured with Kieldahl method. Amino acids constituting the protein were determined by means of acidolysis. The proteins were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and capillary zone electrophoresis (CZE). Results Contents of proteins in the seed of spine date and the seed of Indian jujube were 36.13% and 41.58%, respectively. The content of valine and methionine in the seed of Indian jujube were significantly more than that in the seed of spine date remarkably, and the contents of other amino acids from the seed of spine date and seed of Indian jujube were similar. Their SDS-PAGE spectra showed that the seed of spine date contained a protein with molecular weight 39800 and the seed of Indian jujube contained a protein with molecular weight 50100. In CZE spectra for the seed of spine date, there was a peak at the mobile time of 3.1min and in CZE spectra from the seed of Indian jujube, there was a peak at the mobile time of 4.8min. Conclusion Protein content in the seed of Indian jujube is more than that in the seed of spine date; The content of valine and methionine in the seed of Indian jujube is more than that in the seed of spine date remarkably. The spectra of SDS-PAGE and CZE for the seed of spine date are different from that for the seed of Indian jujube clearly.