白血病患者血清硒、VEGF和sFas水平相关性的初步研究

为了解白血病患者体内具有防癌作用的微量元素硒与促血管新生因子VEGF和凋亡抑制因子sFas的相关性，应用原子荧光法测定白血病患者血清硒浓度，用酶联免疫吸附试验（ELISA）法同步测定血清VEGF和sFas水平，并以直线相关比较硒、VEGF和sFas水平变化的相关性。结果显示：白血病患者硒浓度低于正常人，其中初发和难治／复发组与对照组相比差异有显著性（P〈0.05，P〈0．01）；硒浓度降低尤以难治／复发组明显，与初发组相比亦有显著性差异（P〈0．01）；缓解组与对照组之间差异无统计学意义（P〉0．05）。初发和难治／复发组的VEGF水平明显高于对照组，差异有统计学意义（P〈0．01）；难治／复发组VEGF水平亦显著高于初发组（P〈0．01）；缓解组与对照组无显著性差异（P〉0．05）。初发和难治／复发组的sFas水平明显高于对照组和缓解组（P值均〈0．01），而缓解组与对照组之间无显著性差异（P〉0.05）。白血病患者血清硒浓度与血清VEGF和sFas水平之间均成显著负相关关系（分别为r=-0．529，P〈0．01；r=-0．432，P〈0．01）。血清VEGF与sFas水平之间呈正相关关系（r=0．663，P〈0．01）。结论：硒、VEGF和sFas与白血病细胞耐药有关。硒与VEGF和sFas水平呈显著负相关关系，本研究结果为硒作为化疗辅助用药应用于临床提供了部分实验依据，但仍有待于在更多病例中作系统深入的探索。

Relationships of Serum Selenium,VEGF and sFas Levels in Leukemia Patients

In order to investigate the relationships of cancer chemopreventive trace element-selenium, vascular endothelial growth factor (VEGF) and soluble Fas (sFas) in leukemia patients, serum selenium concentration was measured by atomic spectrometry and levels of VEGF and sFas were simultaneously detected by enzyme linked immunosorbent assay (ELISA). Relationships of selenium, VEGF and sFas were analyzed by linear correlation. The results showed that serum selenium concentration in newly-diagnosed patients and relapsed patients was significantly lower than that in the control group (p < 0.05), especially in relapsed group. VEGF and sFas concentrations of refractory/relapsed group were significantly higher than those in the control group and the remission group (p < 0.05). But no significant difference was found between the remission group and the control group (p > 0.05). In leukemia patients, negative correlation was observed between selenium and VEGF (r = -0.529, p < 0.01), so did between selenium and sFas (r = -0.432, p < 0.01). Positive correlation was found between VEGF and sFas (r = 0.663, p < 0.01). It is concluded that the selenium, VEGF and sFas may take part in the occurrence of MDR in leukemia patients. Selenium has negative correlation with VEGF and sFas, which means that it may be used as an effective assistant agent to improve therapeutic effect of chemotherapy. However, further study in more cases is needed to reach a definite conclusion.