| N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对血小板洐生生长因子介导的NIH3T3细胞增殖的抑制调节作用 |
| |
| 引用本文： | 杨方,赵丹,马文东,罗玲,户万秘,王丽萍,朱曦玲,孙树勋. N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对血小板洐生生长因子介导的NIH3T3细胞增殖的抑制调节作用[J]. 解剖学杂志, 2005, 28(6): 618-620,681 |
| |
| 作者姓名： | 杨方 赵丹 马文东 罗玲 户万秘 王丽萍 朱曦玲 孙树勋 |
| |
| 作者单位： | 华北煤炭医学院实验研究中心,唐山,063000;华北煤炭医学院实验研究中心,唐山,063000;华北煤炭医学院实验研究中心,唐山,063000;华北煤炭医学院实验研究中心,唐山,063000;华北煤炭医学院实验研究中心,唐山,063000;华北煤炭医学院实验研究中心,唐山,063000;华北煤炭医学院实验研究中心,唐山,063000;华北煤炭医学院实验研究中心,唐山,063000 |
| |
| 基金项目： | 河北省科技厅博士基金（04547002D-5）;河北省教育厅基金（2003112）;店山市新医药基础研究重点实验室项目（04362001B） |
| |
| 摘 要： | 目的：探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸（AcSDKP）对血小板洐生生长因子（PDGF）介导的NIH3T3细胞增殖的抑制调节作用。方法：采用MTT法和免疫组化法检测NIH3T3细胞增殖和增殖细胞核抗原（PCNA）的表达。结果：在10^-10～10^-8mol／L浓度范围内。AcSDKP对PDGF介导的NIH3T3细胞增殖均有抑制作用。并在10^-9mol／L浓度时其抑制作用最佳。PDGF对NIH3T3细胞PCNA的表达具有增强作用．而AcSDKP对PDGF介导的NIH3T3细胞PCNA的表达有显著抑制作用。结论：AcSDKP对PDGF介导的NIH3T3细胞增殖有明显抑制作用。
|
| 关 键 词： | N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸 血小板珩生生长因子 增殖细胞核抗原 NIH3T3细胞 细胞增殖 |
Inhibition of N-acetyl-seryl-aspartyl-lysyl-proline on NIH3T3 cells proliferation mediated by platelet-derived growth factor in vitro |
| |
| YANG Fang,ZHAO Dan,MA Wen-Dong,LUO Ling,HU Wan-Mi,WANG Li-Ping,ZHU Xi-Ling,SUN Shu-Xun. Inhibition of N-acetyl-seryl-aspartyl-lysyl-proline on NIH3T3 cells proliferation mediated by platelet-derived growth factor in vitro[J]. Chinese Journal of Anatomy, 2005, 28(6): 618-620,681 |
| |
| Authors: | YANG Fang ZHAO Dan MA Wen-Dong LUO Ling HU Wan-Mi WANG Li-Ping ZHU Xi-Ling SUN Shu-Xun |
| |
| Affiliation: | Experimental and Research Center, North China Coal Medical College, Tangshan 063000, China |
| |
| Abstract: | Objective: To investigate whether N-acetyl-seryl-aspartyMysyl-proline (AcSDKP) can inhibit NIH3T3 cell proliferation mediated by plateletTderived growth factor (PDGF). Methods: Proliferation of NIH3T3 cells and expression of proliferating cells nuclear antigen (PCNA) were measured by MTT assay and immunohistochemistry. Results: AcSDKP at the concentration of 10-10-10-8mol/L inhibited NIH3T3 cells proliferation mediated by PDGF. 10-9 mol/L AcSDKP had the strongest inhibiting effect on NIH3T3 cells proliferation induced by PDGF. PDGF increased expression of PCNA in NIH3T3 cells. However, AcSDKP inhibited this effect mediated by PDGF. Conclusion: AcSDKP can inhibit NIH3T3 cells proliferation mediated by PDGF. |
| |
| Keywords: | N-acetyl-seryl aspartyl lysyl proline platelet derived growth factor proliferating cells nuclear antigen NIH3T3 cells cell proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
